Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Cleaved Caspase-3 antibody [E83-77] - BSA and Azide free (ab208003)

Knockout Tested Rabbit recombinant monoclonal active Caspase-3 antibody [E83-77]. Validated in WB, IHC, ICC/IF and tested in Human. Cited in 1 publication(s).

Overview

  • Product name

    Anti-Cleaved Caspase-3 antibody [E83-77] - BSA and Azide free
    See all Cleaved Caspase-3 primary antibodies
  • Description

    Rabbit monoclonal [E83-77] to Cleaved Caspase-3 - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This antibody only detects the active (cleaved) form of Caspase-3 and does not recognize the pro form of Caspase-3. PubMedID 19789217 describes the detection of human cells injected into mice.
  • Tested applications

    Suitable for: WB, IHC-Fr, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Cleaved Caspase-3 aa 1-100 (N terminal). The exact sequence is proprietary. A synthetic peptide corresponding to residues following Ser29 of human Caspase 3 (N terminus of p17 subunit).
    Database link: P42574

  • Positive control

    • WB: Jurkat cell lysate. IF: Hela cells.
  • General notes

    Ab208003 is the carrier-free version of ab32042. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab208003 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab208003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 17 kDa (predicted molecular weight: 32 kDa).
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
      -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
    • Tissue specificity

      Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
    • Sequence similarities

      Belongs to the peptidase C14A family.
    • Post-translational
      modifications

      Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
      S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • active caspase 3 antibody
      • Apopain antibody
      • CASP 3 antibody
      • CASP-3 antibody
      • CASP3 antibody
      • CASP3_HUMAN antibody
      • Caspase 3 antibody
      • Caspase-3 subunit p12 antibody
      • CPP 32 antibody
      • CPP-32 antibody
      • CPP32B antibody
      • Cysteine protease CPP32 antibody
      • PARP cleavage protease antibody
      • Protein Yama antibody
      • SCA-1 antibody
      • SCA1 antibody
      • SREBP cleavage activity 1 antibody
      • Yama antibody
      see all

    Images

    • Ab32042, at dilution of 1/100, staining HeLa cells by Immunofluorescence.
      Left image: control.
      Right image: staurosporine treated.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32042).

    References

    This product has been referenced in:

    • Xu J  et al. NEDD4 protects vascular endothelial cells against Angiotensin II-induced cell death via enhancement of XPO1-mediated nuclear export. Exp Cell Res 383:111505 (2019). Read more (PubMed: 31326389) »
    • Shen J  et al. Lycorine inhibits glioblastoma multiforme growth through EGFR suppression. J Exp Clin Cancer Res 37:157 (2018). Read more (PubMed: 30016965) »
    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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