Recombinant
RabMAb

Recombinant Anti-Cleaved Caspase-3 antibody [EPR21032] - BSA and Azide free (ab231289)

Overview

  • Product name

    Anti-Cleaved Caspase-3 antibody [EPR21032] - BSA and Azide free
    See all Cleaved Caspase-3 primary antibodies
  • Description

    Rabbit monoclonal [EPR21032] to Cleaved Caspase-3 - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    ab214430 recognises both pro-Caspase-3 and p17 cleavage fragments.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Mouse Cleaved Caspase-3 aa 150-250.
    Database link: P70677

  • Positive control

    • WB: NIH/3T3 cells treated with staurosporine, whole cell lysate.
  • General notes

    Ab231289 is the carrier-free version of ab214430. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab231289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 32, 29, 24, 19, 17 kDa (predicted molecular weight: 31 kDa).

Target

  • Function

    Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
  • Tissue specificity

    Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
  • Sequence similarities

    Belongs to the peptidase C14A family.
  • Post-translational
    modifications

    Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
    S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • active caspase 3 antibody
    • Apopain antibody
    • CASP 3 antibody
    • CASP-3 antibody
    • CASP3 antibody
    • CASP3_HUMAN antibody
    • Caspase 3 antibody
    • Caspase-3 subunit p12 antibody
    • CPP 32 antibody
    • CPP-32 antibody
    • CPP32B antibody
    • Cysteine protease CPP32 antibody
    • PARP cleavage protease antibody
    • Protein Yama antibody
    • SCA-1 antibody
    • SCA1 antibody
    • SREBP cleavage activity 1 antibody
    • Yama antibody
    see all

Images

  • All lanes : Anti-Cleaved Caspase-3 antibody [EPR21032] (ab214430) at 1/5000 dilution

    Lane 1 : Untreated NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 10 µg
    Lane 2 : NIH/3T3 cells treated with 1 uM staurosprine for 4 hours, whole cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 31 kDa
    Observed band size: 14,19,24,29,32 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and dilution buffer: 5% NFDM/TBST.

    Observed molecular masses are consistent with the literature for full-length and cleaved caspase 3 (PMID: 9922454, PMID 16221205).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214430).

     

References

ab231289 has not yet been referenced specifically in any publications.

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