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    cleaved-caspase-3-staining-kit-red-ab65617.pdf

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Cell Biology Apoptosis Intracellular Caspases etc Caspases
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Cleaved Caspase-3 Staining Kit (Red) (ab65617)

  • Datasheet
  • SDS
  • Protocol Booklet
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Functional assays: Caspase-3 (active) Red Staining Kit (ab65617)

    Key features and details

    • Assay type: Enzyme activity
    • Platform: Microplate reader, Fluor. microscope, Flow cyt.
    • Assay time: 2 hr
    • Sample type: Adherent cells, Suspension cells

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    Overview

    • Product name

      Cleaved Caspase-3 Staining Kit (Red)
      See all Caspase-3 kits
    • Sample type

      Adherent cells, Suspension cells
    • Assay type

      Enzyme activity
    • Assay time

      2h 00m
    • Product overview

      Cleaved Caspase-3 Staining Kit (Red) ab65617 provides a convenient means for detecting activated Caspase-3 in living cells. The assay utilizes the Caspase-3 inhibitor DEVD-FMK conjugated to sulfo-rhodamine (Red-DEVD-FMK) as the fluorescent in situ marker. Red-DEVD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-3 in apoptotic cells.

    • Notes

      This product is manufactured by BioVision, an Abcam company and was previously called K193 CaspGLOW™ Red Active Caspase-3 Staining Kit. K193-100 is the same size as the 100 test size of ab65617.

    • Platform

      Microplate reader, Fluor. microscope, Flow cyt.

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 100 tests
      Red-DEVD-FMK 1 x 100µl
      Wash Buffer 2 x 100ml
      Z-VAD-FMK 1 x 10µl
    • Research areas

      • Cell Biology
      • Apoptosis
      • Intracellular
      • Caspases etc
      • Caspases
      • Cancer
      • Invasion/microenvironment
      • Apoptosis
      • Caspases
      • Cell Biology
      • Proteolysis / Ubiquitin
      • Proteolytic enzymes
      • Other proteases
      • Kits/ Lysates/ Other
      • Kits
      • Apoptosis Kits
      • Caspase Assays Kits
      • Caspase 3 assay kits
      • Metabolism
      • Pathways and Processes
      • Metabolism processes
      • Apoptosis
      • Cancer
      • Cell Death
      • Apoptosis
      • Apoptosis Markers
      • Caspases
      • Cancer
      • Cell Death
      • Apoptosis
      • Metabolism
    • Relevance

      The caspase family of cysteine proteases play a key role in apoptosis. Caspase 3 (also known as CPP32, YAMA and apopain) is the most extensively studied apoptotic protein among caspase family members. Caspase 3 is synthesized as an inactive pro enzyme that is processed in cells undergoing apoptosis by self proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10). The processed form of Caspase 3 consists of large (17kD) and small (12kD) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28 - Ser29 and Asp175 - Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Alternative splicing of this gene results in two transcript variants which encode the same protein. In immunohistochemical studies Caspase 3 expression has been shown to be widespread but not present in all cell types (e.g. commonly reported in epithelial cells of skin, renal proximal tubules and collecting ducts). Differences in the level of Caspase 3 have been reported in cells of short lived nature (eg germinal centre B cells) and those that are long lived (e.g. mantle zone B cells). Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.
    • Cellular localization

      Cytoplasmic
    • Alternative names

      • A830040C14Rik
      • Apopain
      • Apopain precursor
      • CASP 3
      • CASP-3
      • CASP3
      • CASP3_HUMAN
      • Casp3a
      • Caspase 3 apoptosis related cysteine protease
      • Caspase 3 p12 subunit
      • Caspase 3 p17 subunit
      • Caspase 3, apoptosis-related cysteine peptidase
      • Caspase 3, apoptosis-related cysteine protease
      • Caspase 3, apoptosis-related cysteine protease a
      • Caspase-3 subunit p12
      • Caspase3
      • CC3
      • CP32B
      • CPP 32
      • CPP-32
      • CPP32
      • CPP32B
      • Cysteine protease CPP32
      • EC 3.4.22.56
      • ICE3
      • LICE
      • mldy
      • OTTHUMP00000165052
      • OTTHUMP00000165053
      • OTTHUMP00000165054
      • PARP cleavage protease
      • Procaspase3
      • Protein Yama
      • SCA 1
      • SCA-1
      • SCA1
      • SREBP cleavage activity 1
      • Yama
      • Yama protein
      see all

    Associated products

    • Related Products

      • Z-D(OMe)E(OMe)VD(OMe)-FMK, Cell permeable caspase-3 inhibitor (ab120488)
      • Z-VDVAD-FMK, caspase-2 inhibitor (ab142035)
      • Q-DEVD-OPh, caspase-3 inhibitor (ab142037)
      • PAC-1, caspase-3 activator (ab142074)

    Images

    • Functional assays: Caspase-3 (active) Red Staining Kit (ab65617)
      Functional assays: Caspase-3 (active) Red Staining Kit (ab65617)

      Caspase-3 activity in Jurkat cells (3 x10e5 cells) following 24 hour exposure to 2 µM Camptothecin (ab120115) with or without 50 μM caspase inhibitor Z-VAD(OMe)-FMK (ab120487). Background signal subtracted, duplicates; +/- SD.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab65617? Please let us know so that we can cite the reference in this datasheet.

    ab65617 has been referenced in 1 publication.

    • Chen YQ  et al. Knockdown of lncRNA TTTY15 alleviates myocardial ischemia-reperfusion injury through the miR-374a-5p/FOXO1 axis. IUBMB Life 73:273-285 (2021). PubMed: 33296140

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