Overview

  • Product name

    Anti-Cleaved Caspase-9 antibody
  • Description

    Rabbit polyclonal to Cleaved Caspase-9
  • Host species

    Rabbit
  • Specificity

    This antibody recognizes only the cleaved caspase-9 (37 kDa). It does not recognize full-length caspase-9 or any other caspase.

  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Positive control

    • WB: Etoposide treated Jurkat whole cell lysate.
  • General notes

    Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 9 is an important member of the caspase family. Upon induction of apoptosis, Cytochrome c released from mitochondria associates with pro-caspase 9 (47 kDa) and Apaf-1. The complex processes pro-caspase 9 into a large subunit (37 kDa/17 kDa) and a small subunit (10 kDa). Cleaved caspase 9 further processes other caspases including caspase 3 and caspase 6, to initiate a caspase cascade leading to apoptosis. This affinity purified antibody recognizing the active forms of caspase 9 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Thimerosal (merthiolate)
    Constituents: PBS, 50% Glycerol, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Primary antibody notes

    Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 9 is an important member of the caspase family. Upon induction of apoptosis, Cytochrome c released from mitochondria associates with pro-caspase 9 (47 kDa) and Apaf-1. The complex processes pro-caspase 9 into a large subunit (37 kDa/17 kDa) and a small subunit (10 kDa). Cleaved caspase 9 further processes other caspases including caspase 3 and caspase 6, to initiate a caspase cascade leading to apoptosis. This affinity purified antibody recognizing the active forms of caspase 9 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 10 - 20 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml.

Target

  • Database links

  • Alternative names

    • active caspase 9 antibody
    • APAF 3 antibody
    • APAF3 antibody
    • Apoptotic protease activating factor 3 antibody
    • Apoptotic protease MCH 6 antibody
    • CASP9 antibody
    • Caspase 9 antibody
    • Caspase 9 apoptosis related cysteine peptidase antibody
    • Cleaved caspase 9 antibody
    • ICE LAP6 antibody
    • ICE like apoptotic protease 6 antibody
    • MCH6 antibody
    • PPP1R56 antibody
    • Protein phosphatase 1 regulatory subunit 56 antibody
    see all

Images

  • All lanes : Anti-Cleaved Caspase-9 antibody (ab2324)

    Lane 1 : Jurkat cell lysate, untreated
    Lane 2 : Jurkat cell lysate, treated with etoposide for 6 hours

    Observed band size: 37 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Chen Z  et al. Protective effects of lncRNA H19 silence against hypoxia-induced injury in PC-12 cells by regulating miR-28. Int J Biol Macromol 121:546-555 (2019). Read more (PubMed: 30312698) »
  • Qi D  et al. Knockdown of lncRNA-H19 inhibits cell viability, migration and invasion while promotes apoptosis via microRNA-143/RUNX2 axis in retinoblastoma. Biomed Pharmacother 109:798-805 (2019). Read more (PubMed: 30551533) »
See all 59 Publications for this product

Customer reviews and Q&As

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1-4 of 4 Abreviews

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Methanol
Permeabilization
No

Dr. Kirk Mcmanus

Verified customer

Submitted Oct 14 2008

Application
Flow Cytometry
Sample
Human Cell (Peripheral blood mononuclear cell)
Permeabilization
Yes - BD Perm/Wash buffer
Gating Strategy
lymphocytes
Specification
Peripheral blood mononuclear cell
Preparation
Cell harvesting/tissue preparation method: Spin down, wash in BD Perm/Wash buffer 1x, fix, wash 2x, and stain with primary antibody 30 minutes.
Sample buffer: Buffer composition at analysis was < 0.09% sodium azide with FBS in phosphate buffered solution.
Fixation
Formaldehyde

Ms. Janine Sabino

Verified customer

Submitted Aug 20 2018

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Tissue lysate - whole (liver)
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Dec 26 2014

Application
Flow Cytometry
Sample
Human Cell (Jurkat)
Specification
Jurkat
Preparation
Cell harvesting/tissue preparation method: Spin down, wash in FACS buffer 1x, fix, wash 2x, and stain with primary antibody overnight.
Sample buffer: Buffer composition at analysis was 0.1% sodium azide with FBS in phosphate buffered solution.
Fixation
Paraformaldehyde
Permeabilization
Yes - Triton X-100 and NP40
Gating Strategy
Isolate cell population from plot of SSC-A / FSA-A

Abcam user community

Verified customer

Submitted Sep 15 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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