Product nameAnti-Cleaved Lamin A + C (Asp230) antibody [EPR4520-11]
See all Cleaved Lamin A + C (Asp230) primary antibodies
DescriptionRabbit monoclonal [EPR4520-11] to Cleaved Lamin A + C (Asp230)
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
corresponding to Human Cleaved Lamin A + C (Asp230) aa 200-300.
- Lysate of NIH/3T3 cells treated with staurosporine, NIH/3T3 cell lysate, lysate of HeLa cells treated with camptothecine, HeLa whole cell lysate (ab150035)
A trial size is available to purchase for this antibody.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133269 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 45-50 kDa (predicted molecular weight: 74 kDa).|
|IP||1/10 - 1/100.|
All lanes : Anti-Cleaved Lamin A + C (Asp230) antibody [EPR4520-11] (ab133269) at 1/50000 dilution
Lane 1 : Lysate of NIH 3T3 cells treated with staurosporine
Lane 2 : NIH 3T3 cell lysate
Lane 3 : Lysate of HeLa cells treated with camptothecine
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 74 kDa
Observed band size: 45-50 kDa why is the actual band size different from the predicted?
Note: The observed bands are possible cleavage fragments.