Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4520-11] to Cleaved Lamin A + C (Asp230) - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-Cleaved Lamin A + C (Asp230) antibody [EPR4520-11] - BSA and Azide free
See all Cleaved Lamin A + C (Asp230) primary antibodies
DescriptionRabbit monoclonal [EPR4520-11] to Cleaved Lamin A + C (Asp230) - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Cleaved Lamin A + C (Asp230) aa 200-300. The exact sequence is proprietary.
Database link: P02545
Ab248435 is the carrier-free version of ab133269. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab248435 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
Our Abpromise guarantee covers the use of ab248435 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 45-50 kDa (predicted molecular weight: 74 kDa).|
All lanes : Anti-Cleaved Lamin A + C (Asp230) antibody [EPR4520-11] (ab133269) at 1/50000 dilution
Lane 1 : Lysate of NIH 3T3 cells treated with staurosporine
Lane 2 : NIH 3T3 cell lysate
Lane 3 : Lysate of HeLa cells treated with camptothecine
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 74 kDa
Observed band size: 45-50 kDa why is the actual band size different from the predicted?
This data was developed using ab133269, the same antibody clone in a different buffer formulation.
Note: The observed bands are possible cleavage fragments.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab248435 has not yet been referenced specifically in any publications.