Recombinant
RabMAb

Recombinant Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160] (ab222407)

Overview

  • Product name

    Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160]
    See all cleaved N-terminal DFNA5 / GSDME primary antibodies
  • Description

    Rabbit monoclonal [EPR20866-160] to cleaved N-terminal DFNA5 / GSDME
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide within Mouse cleaved N-terminal DFNA5/ GSDME aa 250-350. The exact sequence is proprietary.
    Database link: Q9Z2D3

  • Positive control

    • WB: CT26 (treated with 2 µg/ml Actinomycin-D for 24 hours) whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20866-160
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab222407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 35 kDa (predicted molecular weight: 57 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Plays a role in the TP53-regulated cellular response to DNA damage probably by cooperating with TP53 (PubMed:16897187, PubMed:18223688, PubMed:21522185). The N-terminal moiety promotes pyroptosis and exhibits bactericidal activity (PubMed:27281216). The physiological relevance of these observations is unknown.
    • Tissue specificity

      Expressed in cochlea. Low level of expression in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas, with highest expression in placenta.
    • Involvement in disease

      Deafness, autosomal dominant, 5
      Is a tumor suppressor gene with an important role in colorectal cancer (CRC).
    • Sequence similarities

      Belongs to the gasdermin family.
    • Domain

      Intramolecular interactions between N- and C-terminal domains may be important for autoinhibition in the absence of activation signal. The intrinsic pyroptosis-inducing activity is carried by the N-terminal domain.
    • Cellular localization

      Cytoplasm, cytosol. Cell membrane.
    • Information by UniProt
    • Alternative names

      • DFNA5 antibody
      • DFNA5_HUMAN antibody
      • ICERE-1 antibody
      • Inversely correlated with estrogen receptor expression 1 antibody
      • Non-syndromic hearing impairment protein 5 antibody
      see all

    Images

    • All lanes : Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160] (ab222407) at 1/5000 dilution

      Lane 1 : Untreated CT26 (mouse colon carcinoma) whole cell lysate
      Lane 2 : CT26 (treated with 2 µg/ml Actinomycin-D for 24 hours) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 57 kDa
      Observed band size: 35 kDa
      why is the actual band size different from the predicted?


      Exposure time: 103 seconds


      Lysates were kindly provided by Dr Feng Shao.

      The molecular weight observed is consistent with the literature (PMID: 28459430, 30804337).

      Blocking/Dilution buffer: 5% NFDM/TBST.

    References

    ab222407 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab222407.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up