Recombinant Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20867-248] (ab222408)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20867-248] to cleaved N-terminal DFNA5 / GSDME
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20867-248]
See all cleaved N-terminal DFNA5 / GSDME primary antibodies -
Description
Rabbit monoclonal [EPR20867-248] to cleaved N-terminal DFNA5 / GSDME -
Host species
Rabbit -
Specificity
This antibody may weakly cross react with the full length DFNA5/GSDME.
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Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: SH-SY5Y (treated with etoposide (60uM, 14h) whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20867-248 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab222408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 55 kDa).
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Notes |
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WB
1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 55 kDa). |
Target
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Function
Plays a role in the TP53-regulated cellular response to DNA damage probably by cooperating with TP53 (PubMed:16897187, PubMed:18223688, PubMed:21522185). The N-terminal moiety promotes pyroptosis and exhibits bactericidal activity (PubMed:27281216). The physiological relevance of these observations is unknown. -
Tissue specificity
Expressed in cochlea. Low level of expression in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas, with highest expression in placenta. -
Involvement in disease
Deafness, autosomal dominant, 5
Is a tumor suppressor gene with an important role in colorectal cancer (CRC). -
Sequence similarities
Belongs to the gasdermin family. -
Domain
Intramolecular interactions between N- and C-terminal domains may be important for autoinhibition in the absence of activation signal. The intrinsic pyroptosis-inducing activity is carried by the N-terminal domain. -
Cellular localization
Cytoplasm, cytosol. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1687 Human
- SwissProt: O60443 Human
- Unigene: 520708 Human
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Alternative names
- DFNA5 antibody
- DFNA5_HUMAN antibody
- ICERE-1 antibody
see all
Images
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All lanes : Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20867-248] (ab222408) at 1/1000 dilution
Lane 1 : Wild-type SH-SY5Y (human neuroblastoma cell line from bone marrow) (treated with treated with etoposide (60uM, 14h), whole cell lysate
Lane 2 : Untreated wild-type SH-SY5Y whole cell lysate
Lane 3 : DFNA5/GSDME knockout SH-SY5Y treated with etoposide (60 uM, 14h), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 27 secondsBlocking and dilution buffer: 5% NFDM/TBST.
The lysates were kindly provided by our collaborator Dr Feng Shao's lab, NIBS.
The molecular weight observed is consistent with the literature (PMID: 28459430, 30804337).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab222408 has been referenced in 2 publications.
- Zhao Q et al. The central role of a two-way positive feedback pathway in molecular targeted therapies-mediated pyroptosis in anaplastic thyroid cancer. Clin Transl Med 12:e727 (2022). PubMed: 35184413
- Yao F et al. HDAC11 promotes both NLRP3/caspase-1/GSDMD and caspase-3/GSDME pathways causing pyroptosis via ERG in vascular endothelial cells. Cell Death Discov 8:112 (2022). PubMed: 35279683