Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20867-248] (ab222408)

Overview

  • Product name

    Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20867-248]
    See all cleaved N-terminal DFNA5 / GSDME primary antibodies
  • Description

    Rabbit monoclonal [EPR20867-248] to cleaved N-terminal DFNA5 / GSDME
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human cleaved N-terminal DFNA5/ GSDME aa 250-350. The exact sequence is proprietary.
    Database link: O60443

  • Positive control

    • WB: SH-SY5Y (treated with etoposide (60uM, 14h) whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20867-248
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab222408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 55 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Plays a role in the TP53-regulated cellular response to DNA damage probably by cooperating with TP53 (PubMed:16897187, PubMed:18223688, PubMed:21522185). The N-terminal moiety promotes pyroptosis and exhibits bactericidal activity (PubMed:27281216). The physiological relevance of these observations is unknown.
    • Tissue specificity

      Expressed in cochlea. Low level of expression in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas, with highest expression in placenta.
    • Involvement in disease

      Deafness, autosomal dominant, 5
      Is a tumor suppressor gene with an important role in colorectal cancer (CRC).
    • Sequence similarities

      Belongs to the gasdermin family.
    • Domain

      Intramolecular interactions between N- and C-terminal domains may be important for autoinhibition in the absence of activation signal. The intrinsic pyroptosis-inducing activity is carried by the N-terminal domain.
    • Cellular localization

      Cytoplasm, cytosol. Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • DFNA5 antibody
      • DFNA5_HUMAN antibody
      • ICERE-1 antibody
      • Inversely correlated with estrogen receptor expression 1 antibody
      • Non-syndromic hearing impairment protein 5 antibody
      see all

    Images

    • All lanes : Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20867-248] (ab222408) at 1/1000 dilution

      Lane 1 : Wild-type SH-SY5Y (human neuroblastoma cell line from bone marrow) (treated with treated with etoposide (60uM, 14h), whole cell lysate
      Lane 2 : Untreated wild-type SH-SY5Y whole cell lysate
      Lane 3 : DFNA5/GSDME knockout SH-SY5Y treated with etoposide (60 uM, 14h), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 55 kDa
      Observed band size: 35 kDa
      why is the actual band size different from the predicted?


      Exposure time: 27 seconds


      Blocking and dilution buffer: 5% NFDM/TBST.

      Lysates were kindly provided by Dr Feng Shao.

      The molecular weight observed is consistent with the literature (PMID: 28459430, 30804337).

    References

    ab222408 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab222408.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up