Product nameAnti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20867-248]
See all cleaved N-terminal DFNA5 / GSDME primary antibodies
DescriptionRabbit monoclonal [EPR20867-248] to cleaved N-terminal DFNA5 / GSDME
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human cleaved N-terminal DFNA5/ GSDME aa 250-350. The exact sequence is proprietary.
Database link: O60443
- WB: SH-SY5Y (treated with etoposide (60uM, 14h) whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab222408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 55 kDa).|
FunctionPlays a role in the TP53-regulated cellular response to DNA damage probably by cooperating with TP53 (PubMed:16897187, PubMed:18223688, PubMed:21522185). The N-terminal moiety promotes pyroptosis and exhibits bactericidal activity (PubMed:27281216). The physiological relevance of these observations is unknown.
Tissue specificityExpressed in cochlea. Low level of expression in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas, with highest expression in placenta.
Involvement in diseaseDeafness, autosomal dominant, 5
Is a tumor suppressor gene with an important role in colorectal cancer (CRC).
Sequence similaritiesBelongs to the gasdermin family.
DomainIntramolecular interactions between N- and C-terminal domains may be important for autoinhibition in the absence of activation signal. The intrinsic pyroptosis-inducing activity is carried by the N-terminal domain.
Cellular localizationCytoplasm, cytosol. Cell membrane.
- Information by UniProt
- DFNA5 antibody
- DFNA5_HUMAN antibody
- ICERE-1 antibody
All lanes : Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20867-248] (ab222408) at 1/1000 dilution
Lane 1 : Wild-type SH-SY5Y (human neuroblastoma cell line from bone marrow) (treated with treated with etoposide (60uM, 14h), whole cell lysate
Lane 2 : Untreated wild-type SH-SY5Y whole cell lysate
Lane 3 : DFNA5/GSDME knockout SH-SY5Y treated with etoposide (60 uM, 14h), whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 27 seconds
Blocking and dilution buffer: 5% NFDM/TBST.
Lysates were kindly provided by Dr Feng Shao.
The molecular weight observed is consistent with the literature (PMID: 28459430, 30804337).
ab222408 has not yet been referenced specifically in any publications.