Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20867-248] to cleaved N-terminal DFNA5 / GSDME - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Product nameAnti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20867-248] - BSA and Azide free
See all cleaved N-terminal DFNA5 / GSDME primary antibodies
DescriptionRabbit monoclonal [EPR20867-248] to cleaved N-terminal DFNA5 / GSDME - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human DFNA5/GSDME aa 250-350. The exact sequence is proprietary.
Database link: O60443
- WB: SH-SY5Y (treated with etoposide (60uM, 14h) whole cell lysate.
Ab256165 is the carrier-free version of ab222408. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab256165 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab256165 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 35 kDa (predicted molecular weight: 55 kDa).|
FunctionPlays a role in the TP53-regulated cellular response to DNA damage probably by cooperating with TP53 (PubMed:16897187, PubMed:18223688, PubMed:21522185). The N-terminal moiety promotes pyroptosis and exhibits bactericidal activity (PubMed:27281216). The physiological relevance of these observations is unknown.
Tissue specificityExpressed in cochlea. Low level of expression in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas, with highest expression in placenta.
Involvement in diseaseDeafness, autosomal dominant, 5
Is a tumor suppressor gene with an important role in colorectal cancer (CRC).
Sequence similaritiesBelongs to the gasdermin family.
DomainIntramolecular interactions between N- and C-terminal domains may be important for autoinhibition in the absence of activation signal. The intrinsic pyroptosis-inducing activity is carried by the N-terminal domain.
Cellular localizationCytoplasm, cytosol. Cell membrane.
- Information by UniProt
- DFNA5 antibody
- DFNA5_HUMAN antibody
- ICERE-1 antibody
All lanes : Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20867-248] (ab222408) at 1/1000 dilution
Lane 1 : Sample: Wild-type SH-SY5Y (human neuroblastoma cell line from bone marrow) (treated with treated with etoposide (60uM, 14h), whole cell lysate, 20 ug
Lane 2 : Untreated wild-type SH-SY5Y whole cell lysate, 20 ug
Lane 3 : DFNA5/GSDME knockout SH-SY5Y treated with etoposide (60 uM, 14h), whole cell lysate, 20 ug
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 55 kDa
Blocking and dilution buffer: 5% NFDM/TBST.
Lysates were kindly provided by Dr Feng Shao.
The molecular weight observed is consistent with the literature (PMID: 28459430, 30804337).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222408).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab256165 has not yet been referenced specifically in any publications.