Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20867-248] - BSA and Azide free (ab256165)

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Overview

  • Product name

    Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20867-248] - BSA and Azide free
    See all cleaved N-terminal DFNA5 / GSDME primary antibodies

  • Description

    Rabbit monoclonal [EPR20867-248] to cleaved N-terminal DFNA5 / GSDME - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human DFNA5/GSDME aa 250-350. The exact sequence is proprietary.
    Database link: O60443

  • Positive control

    • WB: SH-SY5Y (treated with etoposide (60uM, 14h) whole cell lysate.

  • General notes

    Ab256165 is the carrier-free version of ab222408. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab256165 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20867-248

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab256165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 35 kDa (predicted molecular weight: 55 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

    • Target

    • Function

      Plays a role in the TP53-regulated cellular response to DNA damage probably by cooperating with TP53 (PubMed:16897187, PubMed:18223688, PubMed:21522185). The N-terminal moiety promotes pyroptosis and exhibits bactericidal activity (PubMed:27281216). The physiological relevance of these observations is unknown.

    • Tissue specificity

      Expressed in cochlea. Low level of expression in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas, with highest expression in placenta.

    • Involvement in disease

      Deafness, autosomal dominant, 5
      Is a tumor suppressor gene with an important role in colorectal cancer (CRC).

    • Sequence similarities

      Belongs to the gasdermin family.

    • Domain

      Intramolecular interactions between N- and C-terminal domains may be important for autoinhibition in the absence of activation signal. The intrinsic pyroptosis-inducing activity is carried by the N-terminal domain.

    • Cellular localization

      Cytoplasm, cytosol. Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • DFNA5 antibody
        • DFNA5_HUMAN antibody
        • ICERE-1 antibody
        • Inversely correlated with estrogen receptor expression 1 antibody
        • Non-syndromic hearing impairment protein 5 antibody
        see all

      Images

      • Western blot - Anti-DFNA5/GSDME antibody [EPR20867-248] - BSA and Azide free (ab256165)
        Western blot - Anti-DFNA5/GSDME antibody [EPR20867-248] - BSA and Azide free (ab256165)
        All lanes : Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20867-248] (ab222408) at 1/1000 dilution

        Lane 1 : Sample: Wild-type SH-SY5Y (human neuroblastoma cell line from bone marrow) (treated with treated with etoposide (60uM, 14h), whole cell lysate, 20 ug
        Lane 2 : Untreated wild-type SH-SY5Y whole cell lysate, 20 ug
        Lane 3 : DFNA5/GSDME knockout SH-SY5Y treated with etoposide (60 uM, 14h), whole cell lysate, 20 ug

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 55 kDa



        Blocking and dilution buffer: 5% NFDM/TBST.

        Lysates were kindly provided by Dr Feng Shao.

        The molecular weight observed is consistent with the literature (PMID: 28459430, 30804337).

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222408).

      References

      ab256165 has not yet been referenced specifically in any publications.

      Publishing research using ab256165? Please let us know so that we can cite the reference in this datasheet.

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