Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3830-17] to Cleaved NEAS (Asp1185) - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Human
Product nameAnti-Cleaved NEAS (Asp1185) antibody [EPR3830-17] - BSA and Azide free
See all Cleaved NEAS (Asp1185) primary antibodies
DescriptionRabbit monoclonal [EPR3830-17] to Cleaved NEAS (Asp1185) - BSA and Azide free
Only detects the large fragment of Alpha Fodrin resulting from cleavage at aspartic acid 1185.
Tested applicationsSuitable for: IP, WBmore details
Unsuitable for: Flow Cyt,ICC or IHC-P
Species reactivityReacts with: Human
Synthetic peptide within Human Cleaved NEAS (Asp1185) aa 1150-1250. The exact sequence is proprietary.
ab247550 is the carrier-free version of ab92373 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab247550 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as Alpha Fodrin, Cleaved alpha-fodrin (Asp1185)
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferConstituent: PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab247550 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 285 kDa.|
FunctionFodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
Involvement in diseaseEpileptic encephalopathy, early infantile, 5
Sequence similaritiesBelongs to the spectrin family.
Contains 3 EF-hand domains.
Contains 1 SH3 domain.
Contains 23 spectrin repeats.
modificationsPhosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro.
Cellular localizationCytoplasm, cytoskeleton. Cytoplasm, cell cortex. Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells.
- Information by UniProt
- Alpha-II spectrin antibody
- Fodrin alpha chain antibody
- non-erythrocytic 1 antibody
All lanes : Anti-Cleaved NEAS (Asp1185) antibody [EPR3830-17] (ab92373)
Lane 1 : HeLa + Camptothecin cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat + Staurosporine cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 285 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
This data was developed using ab92373, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab247550 has not yet been referenced specifically in any publications.