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Synthetic peptide within Human Cleaved PARP1 aa 200-300 (N terminal). The exact sequence is proprietary.
Database link: P09874
Product was previously marketed under the MitoSciences sub-brand.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or firstname.lastname@example.org
Our Abpromise guarantee covers the use of ab170171 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use a concentration of 1 µg/ml.
Use Antigen Retrieval Buffer (100 mM Tris, 5% urea, pH 9.5) at 95°C for 10 min to boost signal.
|Flow Cyt||Use a concentration of 1 µg/ml. ab171463-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
Immunocytochemistry with anti-cleaved PARP1 antibody conjugated to Alexa Fluor® 488.
HeLa cells were vehicle-treated (panels A-C) or treated with 1 µM staurosporine for 4 hours (panels D-F), then fixed. Cells were treated with antigen retrieval buffer (100 mM Tris, 5% urea, pH 9.5) for 10 minutes at 95°C, then permeabilized and blocked. Cells were incubated with 1 µg/mL of the cleaved PARP1 antibody conjugated to Alexa Fluor® 488, then co-stained with the DNA stain DAPI. Images of DAPI signals (A and D), anti-cleaved PARP1 signal (B and E), and overlays of DAPI (artificially colored red for better contrast) and anti-cleaved PARP1 (colored green) images (C and F) are shown.
Flow cytometry with anti-cleaved PARP1 antibody conjugated to Alexa Fluor® 488.
Flow cytometric analysis was performed on HeLa vehicle-treated cells and on HeLa cells treated with 1 µM staurosporine for 4 hours. Cells were fixed with paraformaldehyde and permeablized with methanol. HeLa vehicle-treated cells were stained with 1 µg/mL of the cleaved PARP1 antibody conjugated to Alexa488 (blue) or a negative, nonreactive Alexa Fluor® 488-conjugated control antibody (black). HeLa staurosporine-treated cells were stained with 1 µg/mL of the cleaved PARP1 antibody conjugated to Alexa Fluor® 488 (yellow) or a negative, nonreactive Alexa Fluor® 488-conjugated control antibody (red). 1% BSA in PBS was used as the blocking reagent for all blocking and antibody incubation steps.
ab170171 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"