Anti-Cleaved PARP1 antibody [4B5BD2] (Alexa Fluor® 488) (ab170171)

Overview

  • Product name
    Anti-Cleaved PARP1 antibody [4B5BD2] (Alexa Fluor® 488)
    See all Cleaved PARP1 primary antibodies
  • Description
    Mouse monoclonal [4B5BD2] to Cleaved PARP1 (Alexa Fluor® 488)
  • Host species
    Mouse
  • Conjugation
    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications
    Suitable for: ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chinese hamsterDoes not react with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide within Human Cleaved PARP1 aa 200-300 (N terminal). The exact sequence is proprietary.
    Database link: P09874

  • Positive control
    • HeLa cells treated with 1 µM staurosporine for 4 hours.
  • General notes

    Product was previously marketed under the MitoSciences sub-brand.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

Our Abpromise guarantee covers the use of ab170171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 1 µg/ml.

Use Antigen Retrieval Buffer (100 mM Tris, 5% urea, pH 9.5) at 95°C for 10 min to boost signal.

Flow Cyt Use a concentration of 1 µg/ml. ab171463-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites.
  • Sequence similarities
    Contains 1 BRCT domain.
    Contains 1 PARP alpha-helical domain.
    Contains 1 PARP catalytic domain.
    Contains 2 PARP-type zinc fingers.
  • Post-translational
    modifications
    Phosphorylated by PRKDC and TXK.
    Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
    S-nitrosylated, leading to inhibit transcription regulation activity.
  • Cellular localization
    Nucleus. Nucleus, nucleolus. Localizes at sites of DNA damage.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADP-ribosyltransferase diphtheria toxin-like 1 antibody
    • ADPRT 1 antibody
    • ADPRT antibody
    • ADPRT1 antibody
    • APOPAIN antibody
    • ARTD1 antibody
    • NAD(+) ADP-ribosyltransferase 1 antibody
    • PARP antibody
    • PARP-1 antibody
    • PARP1 antibody
    • PARP1_HUMAN antibody
    • Poly [ADP-ribose] polymerase 1 antibody
    • Poly ADP ribose polymerase 1 antibody
    • Poly[ADP-ribose] synthase 1 antibody
    • PPOL antibody
    • SCA1 antibody
    see all

Images

  • Immunocytochemistry with anti-cleaved PARP1 antibody conjugated to Alexa Fluor® 488.

    HeLa cells were vehicle-treated (panels A-C) or treated with 1 µM staurosporine for 4 hours (panels D-F), then fixed. Cells were treated with antigen retrieval buffer (100 mM Tris, 5% urea, pH 9.5) for 10 minutes at 95°C, then permeabilized and blocked. Cells were incubated with 1 µg/mL of the cleaved PARP1 antibody conjugated to Alexa Fluor® 488, then co-stained with the DNA stain DAPI. Images of DAPI signals (A and D), anti-cleaved PARP1 signal (B and E), and overlays of DAPI (artificially colored red for better contrast) and anti-cleaved PARP1 (colored green) images (C and F) are shown.

  • Flow cytometry with anti-cleaved PARP1 antibody conjugated to Alexa Fluor® 488.

    Flow cytometric analysis was performed on HeLa vehicle-treated cells and on HeLa cells treated with 1 µM staurosporine for 4 hours. Cells were fixed with paraformaldehyde and permeablized with methanol. HeLa vehicle-treated cells were stained with 1 µg/mL of the cleaved PARP1 antibody conjugated to Alexa488 (blue) or a negative, nonreactive Alexa Fluor® 488-conjugated control antibody (black). HeLa staurosporine-treated cells were stained with 1 µg/mL of the cleaved PARP1 antibody conjugated to Alexa Fluor® 488 (yellow) or a negative, nonreactive Alexa Fluor® 488-conjugated control antibody (red). 1% BSA in PBS was used as the blocking reagent for all blocking and antibody incubation steps.

References

ab170171 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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