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  1. Link

    cleaved-parp1-antibody-e51-ab32064.pdf

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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Cleaved PARP1 antibody [E51] (ab32064)

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  • SDS
Reviews (18)Q&A (4)References (274)

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Western blot - Anti-Cleaved PARP1 antibody [E51] (ab32064)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cleaved PARP1 antibody [E51] (ab32064)
  • Western blot - Anti-Cleaved PARP1 antibody [E51] (ab32064)
  • Western blot - Anti-Cleaved PARP1 antibody [E51] (ab32064)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cleaved PARP1 antibody [E51] (ab32064)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cleaved PARP1 antibody [E51] (ab32064)
  • Western blot - Anti-Cleaved PARP1 antibody [E51] (ab32064)
  • Western blot - Anti-Cleaved PARP1 antibody [E51] (ab32064)
  • Western blot - Anti-Cleaved PARP1 antibody [E51] (ab32064)
  • Western blot - Anti-Cleaved PARP1 antibody [E51] (ab32064)
  • Anti-Cleaved PARP1 antibody [E51] (ab32064)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E51] to Cleaved PARP1
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Knockout
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Human PARP1 knockout HEK-293T cell line (ab266598)
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Anti-PARP1 antibody [E102] (ab32138)

View more associated products

Overview

  • Product name

    Anti-Cleaved PARP1 antibody [E51]
    See all Cleaved PARP1 primary antibodies
  • Description

    Rabbit monoclonal [E51] to Cleaved PARP1
  • Host species

    Rabbit
  • Specificity

    This antibody is specific for the p25 cleaved form of human PARP1.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chinese hamster
  • Immunogen

    Synthetic peptide within Human Cleaved PARP1 aa 150-250. The exact sequence is proprietary.
    Database link: P09874

  • Positive control

    • WB: Jurkat whole cell lysate (ab7899). HeLa and RAW 264.7 whole cell lysate. HAP1, HeLa, NIH/3T3 and PC-12 treated with 1uM Staurosporine. Jukat cells treated with camptothecin. Jukat cells treated with 15-Acetoxyscirpenol. IHC-P: Rat colon tissue. Human ovarian cancer and breast carcinoma tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    E51
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Nucleus
    • PARP
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • ADP-ribosylation
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • DNA Damage Recognition
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • Other
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Apoptosis
    • Nuclear
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Nucleus
    • PARP
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Alternative Versions

    • Anti-Cleaved PARP1 antibody [E51] - BSA and Azide free (ab203467)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Jurkat whole cell lysate (ab7899)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab32064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (7)
1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
IHC-P (5)
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Application notes
Is unsuitable for ICC/IF.

Target

  • Function

    Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites.
  • Sequence similarities

    Contains 1 BRCT domain.
    Contains 1 PARP alpha-helical domain.
    Contains 1 PARP catalytic domain.
    Contains 2 PARP-type zinc fingers.
  • Post-translational
    modifications

    Phosphorylated by PRKDC and TXK.
    Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
    S-nitrosylated, leading to inhibit transcription regulation activity.
  • Cellular localization

    Nucleus. Nucleus, nucleolus. Localizes at sites of DNA damage.
  • Target information above from: UniProt accession P09874 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 100689463 Chinese hamster
    • Entrez Gene: 142 Human
    • Entrez Gene: 11545 Mouse
    • Entrez Gene: 25591 Rat
    • Omim: 173870 Human
    • SwissProt: Q9R152 Chinese hamster
    • SwissProt: P09874 Human
    • SwissProt: P11103 Mouse
    • SwissProt: P27008 Rat
    • Unigene: 177766 Human
    • Unigene: 277779 Mouse
    • Unigene: 11327 Rat
    see all
  • Alternative names

    • ADP ribosyltransferase diphtheria toxin like 1 antibody
    • ADP ribosyltransferase NAD(+) antibody
    • ADP-ribosyltransferase diphtheria toxin-like 1 antibody
    • ADPRT 1 antibody
    • ADPRT antibody
    • ADPRT1 antibody
    • APOPAIN antibody
    • ARTD1 antibody
    • NAD(+) ADP-ribosyltransferase 1 antibody
    • PARP antibody
    • PARP-1 antibody
    • PARP1 antibody
    • PARP1_HUMAN antibody
    • Poly [ADP-ribose] polymerase 1 (PARP-1) antibody
    • Poly [ADP-ribose] polymerase 1 antibody
    • Poly ADP ribose polymerase 1 antibody
    • Poly(ADP ribose) polymerase antibody
    • Poly[ADP-ribose] synthase 1 antibody
    • PPOL antibody
    • SCA1 antibody
    see all

Images

  • Western blot - Anti-Cleaved PARP1 antibody [E51] (ab32064)
    Western blot - Anti-Cleaved PARP1 antibody [E51] (ab32064)
    All lanes : Anti-Cleaved PARP1 antibody [E51] (ab32064) at 1/10000 dilution

    Lane 1 : Wild-type (1uM Staurosporine for 3hrs) HAP1 cell lysate
    Lane 2 : Wild-type (Staurosporine control) HAP1 cell lysate
    Lane 3 : PARP1 knockout (1uM Staurosporine for 3hrs) HAP1 cell lysate
    Lane 4 : PARP1 knockout (Staurosporine control) HAP1 cell lysate
    Lane 5 : HeLa (1uM Staurosporine for 3hrs) cell lysate
    Lane 6 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 25 kDa
    Observed band size: 27 kDa why is the actual band size different from the predicted?



    Lanes 1 - 6: Merged signal (red and green). Green - ab32064 observed at 27 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

    ab32064 was shown to react with Cleaved PARP1 in wild-type HAP1 cells in Western blot with loss of signal observed in PARP1 knockout sample.Wild-type HAP1 and PARP1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab32064 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cleaved PARP1 antibody [E51] (ab32064)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cleaved PARP1 antibody [E51] (ab32064)

    Immunohistochemical staining of paraffin embedded rat colon with purified ab32064 at a working dilution of 1/100. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counterstained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

    PBS was used instead of the primary antibody as the negative control (inset).

  • Western blot - Anti-Cleaved PARP1 antibody [E51] (ab32064)
    Western blot - Anti-Cleaved PARP1 antibody [E51] (ab32064)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: PARP1 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: MCF7 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab32064 observed at 30 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab32064 was shown to specifically react with PARP1 when PARP1 knockout samples were used. Wild-type and PARP1 knockout samples were subjected to SDS-PAGE. Ab32064 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilutions.

    Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) ab216773 and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Cleaved PARP1 antibody [E51] (ab32064)
    Western blot - Anti-Cleaved PARP1 antibody [E51] (ab32064)
    All lanes : Anti-Cleaved PARP1 antibody [E51] (ab32064) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysates treated with 1uM Staurosporine for 3 hours
    Lane 2 : Untreated HeLa whole cell lysates
    Lane 3 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysates treated with 1uM Staurosporine for 3 hours
    Lane 4 : Untreated NIH/3T3 whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 25 kDa
    Observed band size: 25 kDa



    Blocking/Dilution buffer 5% NFDM/TBST

    Exposure time :
    Lane 1,2: 1 second
    Lane 3,4: 8 seconds

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cleaved PARP1 antibody [E51] (ab32064)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cleaved PARP1 antibody [E51] (ab32064)

    Immunohistochemical staining of paraffin embedded human breast carcinoma tissue with unpurified ab32064 at a 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cleaved PARP1 antibody [E51] (ab32064)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cleaved PARP1 antibody [E51] (ab32064)

    Immunohistochemical staining of paraffin embedded human ovarian carcinoma with purified ab32064 at a working dilution of 1 in 100. The secondary antibody used is a HRP polymer for rabbit IgG. Counterstained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

    PBS was used instead of the primary antibody as the negative control (inset).

  • Western blot - Anti-Cleaved PARP1 antibody [E51] (ab32064)
    Western blot - Anti-Cleaved PARP1 antibody [E51] (ab32064)
    All lanes : Anti-Cleaved PARP1 antibody [E51] (ab32064) at 1/10000 dilution

    Lane 1 : Untreated Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
    Lane 2 : Jurkat cell lysate treated with camptothecin

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 25 kDa
    Observed band size: 25 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-Cleaved PARP1 antibody [E51] (ab32064)
    Western blot - Anti-Cleaved PARP1 antibody [E51] (ab32064)
    All lanes : Anti-Cleaved PARP1 antibody [E51] (ab32064) at 1/1000 dilution

    Lane 1 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate
    Lane 2 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 25 kDa
    Observed band size: 25 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Cleaved PARP1 antibody [E51] (ab32064)
    Western blot - Anti-Cleaved PARP1 antibody [E51] (ab32064)
    All lanes : Anti-Cleaved PARP1 antibody [E51] (ab32064) at 1/1000 dilution

    Lane 1 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysates treated with 1uM Staurosporine for 3 hours
    Lane 2 : Untreated PC-12 whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 25 kDa
    Observed band size: 25 kDa


    Exposure time: 30 seconds


    Blockinng/Diluting buffer 5% NFDM/TBST

  • Western blot - Anti-Cleaved PARP1 antibody [E51] (ab32064)
    Western blot - Anti-Cleaved PARP1 antibody [E51] (ab32064)

    Jurkat (Human T cell leukemia cell line from peripheral blood) cells were incubated at 37°C for 24 hours with vehicle control (0 μM) and different concentrations of 15-Acetoxyscirpenol (ab142381). Increased expression of cleaved PARP1 (ab32064) in Jurkat cells correlates with an increase in 15-Acetoxyscirpenol concentration, as described in literature.

    Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab32064 at 1/10000 dilution and ab8227 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 and visualised using ECL development solution.

  • Anti-Cleaved PARP1 antibody [E51] (ab32064)
    Anti-Cleaved PARP1 antibody [E51] (ab32064)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (274)

Publishing research using ab32064? Please let us know so that we can cite the reference in this datasheet.

ab32064 has been referenced in 274 publications.

  • Li J  et al. METTL3 promotes prostatic hyperplasia by regulating PTEN expression in an m6A-YTHDF2-dependent manner. Cell Death Dis 13:723 (2022). WB ; Human . PubMed: 35985997
  • Wu H  et al. miR-34a in extracellular vesicles from bone marrow mesenchymal stem cells reduces rheumatoid arthritis inflammation via the cyclin I/ATM/ATR/p53 axis. J Cell Mol Med 25:1896-1910 (2021). PubMed: 33465281
  • Wu D  et al. Long non-coding RNA GClnc1 knockdown suppresses progression of epithelial ovarian cancer by recruiting FOXC2 to disrupt the NOTCH1/NF-?B/Snail pathway. Exp Cell Res 399:112422 (2021). PubMed: 33338479
  • Wang L  et al. Circ_0000396 inhibits rheumatoid arthritis synovial fibroblast growth and inflammatory response via miR-203/HBP1 axis. J Biol Res (Thessalon) 28:1 (2021). PubMed: 33407952
  • Yu S  et al. An Experimentally Induced Mutation in the UBA Domain of p62 Changes the Sensitivity of Cisplatin by Up-Regulating HK2 Localisation on the Mitochondria and Increasing Mitophagy in A2780 Ovarian Cancer Cells. Int J Mol Sci 22:N/A (2021). PubMed: 33924293
View all Publications for this product

Customer reviews and Q&As

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1-10 of 22 Abreviews or Q&A

Western blot abreview for Anti-Cleaved PARP1 antibody [E51]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HCT116)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
20 µg
Specification
HCT116
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Tae-Won Jang

Verified customer

Submitted Apr 06 2021

Immunohistochemistry (Frozen sections) abreview for Anti-Cleaved PARP1 antibody [E51]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Brain)
Permeabilization
Yes - 0.1% triton
Specification
Brain
Blocking step
Goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 02 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cleaved PARP1 antibody [E51]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (xenograft tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
xenograft tumor
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% normal buffered formalin
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 16 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cleaved PARP1 antibody [E51]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Mesenchymal tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
Mesenchymal tumor
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% formalin
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 16 2019

Western blot abreview for Anti-Cleaved PARP1 antibody [E51]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Lung)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
50 µg
Specification
Lung
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 07 2019

Western blot abreview for Anti-Cleaved PARP1 antibody [E51]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
50 µg
Treatment
500 mg/kg acetaminophen
Specification
Liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MS. Ji Eun Moon

Verified customer

Submitted Apr 29 2019

Western blot abreview for Anti-Cleaved PARP1 antibody [E51]

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (mouse RPE, spleen)
Gel Running Conditions
Reduced Denaturing (12% Bis-Tris gel)
Loading amount
20 µg
Specification
mouse RPE, spleen
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 13 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cleaved PARP1 antibody [E51]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate buffer pH6
Permeabilization
No
Specification
lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
10% formalin
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 19 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cleaved PARP1 antibody [E51]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
Liver
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
10% normal buffered formalin
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 03 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-Cleaved PARP1 antibody [E51]

Good
Abreviews
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Pancreasic islet)
Permeabilization
Yes - Triton 0.2%
Specification
Pancreasic islet
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formalin
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Juan Moreno

Verified customer

Submitted Jun 19 2018

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