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What antigen retrieval was used for the breast cancer IHC done at 1:100 in the picture on the datasheet?
Asked on May 02 2014
The antigen retrieval was heat-mediated with citrate buffer, pH 6.0. The laboratory uses a pressure cooker but a microwave, steamer, or waterbath should work too.
Abcam Scientific Support
Answered on May 02 2014