Non-Abcam antibody was used: donkey anti-Rabbit IgG (H+L) Cross Adsorbed Second Host species: Goat Clonality: Polyclonal Conjugation: Horse Radish Peroxidase
10 minute(s) and 0 second(s)
Specific: 25 kDa Non-specific: 10, 12 kDa
human HEK293T cells untreated and treated with doxorubinin (lanes
We have tried additional conditions: dilution 1:10000, buffer without BSA, dilution 1:1000 (PBST 2%BSA)
We could not find the conditions for this antibody to give reproducible results for zebrafish.
For human samples works well.
Lane 1. HEK297 untreated
Lane 2. HEK297 treated doxorubicin
Lane 3. Zebrafish whole body lysate untreated
Lane 4. Zebrafish whole body lysate treated tamoxifen
Lane 5. Zebrafish whole body lysate treated tamoxifen and cisplatin
Abcam user community
Submitted Jul 28 2015
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