Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cleaved PARP1 antibody [E51]

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Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate buffer pH6
Permeabilization
No
Specification
lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
10% formalin

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: CY3 conjugated donkey anti-rabbit secondary antibo
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®

Additional data

Additional Notes
In the picture below, Cleaved PARP1 staining is represented in red and nuclear staining by Hoechst 33342 is represented in blue.

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Submitted Oct 19 2018

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