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Abcam has not validated the combination of species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Pig Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate buffer pH6
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
16 hour(s) and 0 minute(s) · Temperature: 4°C
Non-Abcam antibody was used: CY3 conjugated donkey anti-rabbit secondary antibo
Host species: Donkey
Conjugation: Cy3 ®
In the picture below, Cleaved PARP1 staining is represented in red and nuclear staining by Hoechst 33342 is represented in blue.
Abcam user community
Submitted Oct 19 2018