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    cleaved-parp1-antibody-sp276-ab225715.pdf

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Cell Biology Apoptosis Nucleus PARP
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Cleaved PARP1 antibody [SP276] (ab225715)

  • Datasheet
  • SDS
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Western blot - Anti-Cleaved PARP1 antibody [SP276] (ab225715)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cleaved PARP1 antibody [SP276] (ab225715)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cleaved PARP1 antibody [SP276] (ab225715)
  • Western blot - Anti-Cleaved PARP1 antibody [SP276] (ab225715)
  • Anti-Cleaved PARP1 antibody [SP276] (ab225715)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP276] to Cleaved PARP1
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-Cleaved PARP1 antibody [SP276]
    See all Cleaved PARP1 primary antibodies
  • Description

    Rabbit monoclonal [SP276] to Cleaved PARP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human tonsil and bladder transitional cell carcinoma tissues. WB: NIH/3T3 cell lysate treated with staurosporine.
  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from TCS by Protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    SP276
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Nucleus
    • PARP
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • ADP-ribosylation
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • DNA Damage Recognition
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Apoptosis
    • Nuclear
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Cell cycle proteins ELISA kits
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • DNA Damage ELISA kits
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Nucleus
    • PARP
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Alternative Versions

    • Anti-Cleaved PARP1 antibody [SP276] - BSA and Azide free (ab272005)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab225715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Primary incubation for 10 minutes at room temperature.

WB
1/100. Predicted molecular weight: 113 kDa.

Primary incubation for 1 hour at room temperature.

Notes
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Primary incubation for 10 minutes at room temperature.

WB
1/100. Predicted molecular weight: 113 kDa.

Primary incubation for 1 hour at room temperature.

Target

  • Function

    Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites.
  • Sequence similarities

    Contains 1 BRCT domain.
    Contains 1 PARP alpha-helical domain.
    Contains 1 PARP catalytic domain.
    Contains 2 PARP-type zinc fingers.
  • Post-translational
    modifications

    Phosphorylated by PRKDC and TXK.
    Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
    S-nitrosylated, leading to inhibit transcription regulation activity.
  • Cellular localization

    Nucleus. Nucleus, nucleolus. Localizes at sites of DNA damage.
  • Target information above from: UniProt accession P09874 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 142 Human
    • Entrez Gene: 11545 Mouse
    • Entrez Gene: 25591 Rat
    • Omim: 173870 Human
    • SwissProt: P09874 Human
    • SwissProt: P11103 Mouse
    • SwissProt: P27008 Rat
    • Unigene: 177766 Human
    • Unigene: 277779 Mouse
    • Unigene: 11327 Rat
    see all
  • Alternative names

    • ADP ribosyltransferase diphtheria toxin like 1 antibody
    • ADP ribosyltransferase NAD(+) antibody
    • ADP-ribosyltransferase diphtheria toxin-like 1 antibody
    • ADPRT 1 antibody
    • ADPRT antibody
    • ADPRT1 antibody
    • APOPAIN antibody
    • ARTD1 antibody
    • NAD(+) ADP-ribosyltransferase 1 antibody
    • PARP antibody
    • PARP-1 antibody
    • PARP1 antibody
    • PARP1_HUMAN antibody
    • Poly [ADP-ribose] polymerase 1 (PARP-1) antibody
    • Poly [ADP-ribose] polymerase 1 antibody
    • Poly ADP ribose polymerase 1 antibody
    • Poly(ADP ribose) polymerase antibody
    • Poly[ADP-ribose] synthase 1 antibody
    • PPOL antibody
    • SCA1 antibody
    see all

Images

  • Western blot - Anti-Cleaved PARP1 antibody [SP276] (ab225715)
    Western blot - Anti-Cleaved PARP1 antibody [SP276] (ab225715)
    All lanes : Anti-Cleaved PARP1 antibody [SP276] (ab225715) at 1/100 dilution

    Lane 1 : Wild-type HAP1 Treated Staurosporine (1 uM, 3 h) cell lysate
    Lane 2 : PARP1 knockout HAP1 Treated Staurosporine (1 uM, 3 h) cell lysate
    Lane 3 : Wild-type HAP1 Control cell lysate
    Lane 4 : PARP1 knockout HAP1 Control cell lysate
    Lane 5 : HeLa Treated Staurosporine (1 uM, 3 h) cell lysate
    Lane 6 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 113 kDa
    Observed band size: 130, 27 kDa why is the actual band size different from the predicted?



    False colour image of Western blot: Anti-Cleaved PARP1 antibody [SP276] staining at 1/100 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab225715 was shown to bind specifically to Cleaved PARP1. A band was observed at 130 (full-length) and 27 (cleaved) kDa in treated wild-type HAP1 cell lysates with no signal observed at this size in PARP1 knockout cell line. To generate this image, wild-type and PARP1 knockout HAP1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cleaved PARP1 antibody [SP276] (ab225715)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cleaved PARP1 antibody [SP276] (ab225715)

    Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for Cleaved PARP1 using ab225715 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cleaved PARP1 antibody [SP276] (ab225715)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cleaved PARP1 antibody [SP276] (ab225715)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for Cleaved PARP1 using ab225715 at 1/100 dilution in immunohistochemical analysis.

  • Western blot - Anti-Cleaved PARP1 antibody [SP276] (ab225715)
    Western blot - Anti-Cleaved PARP1 antibody [SP276] (ab225715)
    All lanes : Anti-Cleaved PARP1 antibody [SP276] (ab225715) at 1/100 dilution

    Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate treated with staurosporine
    Lane 2 : Untreated NIH/3T3 (mouse embryo fibroblast cell line) cell lysate

    Predicted band size: 113 kDa

  • Anti-Cleaved PARP1 antibody [SP276] (ab225715)
    Anti-Cleaved PARP1 antibody [SP276] (ab225715)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab225715? Please let us know so that we can cite the reference in this datasheet.

ab225715 has been referenced in 1 publication.

  • Tutuska K  et al. Statin as anti-cancer therapy in autochthonous T-lymphomas expressing stabilized gain-of-function mutant p53 proteins. Cell Death Dis 11:274 (2020). PubMed: 32332697

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