Recombinant
RabMAb

Recombinant Anti-Cleaved PARP1 antibody [SP276] (ab225715)

Overview

  • Product name

    Anti-Cleaved PARP1 antibody [SP276]
    See all Cleaved PARP1 primary antibodies
  • Description

    Rabbit monoclonal [SP276] to Cleaved PARP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Cleaved PARP1 aa 200-300. The exact sequence is proprietary. (Surrounding Asp 214).
    Database link: P09874

  • Positive control

    • IHC-P: Human tonsil and bladder transitional cell carcinoma tissues. WB: NIH/3T3 cell lysate treated with staurosporine.

Properties

Applications

Our Abpromise guarantee covers the use of ab225715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Primary incubation for 10 minutes at room temperature.

WB 1/100. Predicted molecular weight: 113 kDa.

Primary incubation for 1 hour at room temperature.

Target

  • Function

    Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites.
  • Sequence similarities

    Contains 1 BRCT domain.
    Contains 1 PARP alpha-helical domain.
    Contains 1 PARP catalytic domain.
    Contains 2 PARP-type zinc fingers.
  • Post-translational
    modifications

    Phosphorylated by PRKDC and TXK.
    Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
    S-nitrosylated, leading to inhibit transcription regulation activity.
  • Cellular localization

    Nucleus. Nucleus, nucleolus. Localizes at sites of DNA damage.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADP ribosyltransferase diphtheria toxin like 1 antibody
    • ADP ribosyltransferase NAD(+) antibody
    • ADP-ribosyltransferase diphtheria toxin-like 1 antibody
    • ADPRT 1 antibody
    • ADPRT antibody
    • ADPRT1 antibody
    • APOPAIN antibody
    • ARTD1 antibody
    • NAD(+) ADP-ribosyltransferase 1 antibody
    • PARP antibody
    • PARP-1 antibody
    • PARP1 antibody
    • PARP1_HUMAN antibody
    • Poly [ADP-ribose] polymerase 1 (PARP-1) antibody
    • Poly [ADP-ribose] polymerase 1 antibody
    • Poly ADP ribose polymerase 1 antibody
    • Poly(ADP ribose) polymerase antibody
    • Poly[ADP-ribose] synthase 1 antibody
    • PPOL antibody
    • SCA1 antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for Cleaved PARP1 using ab225715 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for Cleaved PARP1 using ab225715 at 1/100 dilution in immunohistochemical analysis.

  • All lanes : Anti-Cleaved PARP1 antibody [SP276] (ab225715) at 1/100 dilution

    Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate treated with staurosporine
    Lane 2 : Untreated NIH/3T3 (mouse embryo fibroblast cell line) cell lysate

    Predicted band size: 113 kDa

References

ab225715 has not yet been referenced specifically in any publications.

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