Recombinant Anti-Cleaved PARP1 antibody [SP276] (ab225715)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Cleaved PARP1 antibody [SP276]
See all Cleaved PARP1 primary antibodies -
Description
Rabbit monoclonal [SP276] to Cleaved PARP1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human Cleaved PARP1 aa 200-300. The exact sequence is proprietary. (Surrounding Asp 214).
Database link: P09874 -
Positive control
- IHC-P: Human tonsil and bladder transitional cell carcinoma tissues. WB: NIH/3T3 cell lysate treated with staurosporine.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from TCS by Protein A/G. -
Clonality
Monoclonal -
Clone number
SP276 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab225715 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Primary incubation for 10 minutes at room temperature. |
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| WB | 1/100. Predicted molecular weight: 113 kDa. Primary incubation for 1 hour at room temperature. |
Target
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Function
Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites. -
Sequence similarities
Contains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 2 PARP-type zinc fingers. -
Post-translational
modificationsPhosphorylated by PRKDC and TXK.
Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
S-nitrosylated, leading to inhibit transcription regulation activity. -
Cellular localization
Nucleus. Nucleus, nucleolus. Localizes at sites of DNA damage. - Information by UniProt
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Database links
- Entrez Gene: 142 Human
- Entrez Gene: 11545 Mouse
- Entrez Gene: 25591 Rat
- Omim: 173870 Human
- SwissProt: P09874 Human
- SwissProt: P11103 Mouse
- SwissProt: P27008 Rat
- Unigene: 177766 Human
see all -
Alternative names
- ADP ribosyltransferase diphtheria toxin like 1 antibody
- ADP ribosyltransferase NAD(+) antibody
- ADP-ribosyltransferase diphtheria toxin-like 1 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cleaved PARP1 antibody [SP276] (ab225715)](https://www.abcam.com/ps/products/225/ab225715/Images/ab225715-286625-anti-cleaved-parp1-antibody-sp276-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cleaved PARP1 antibody [SP276] (ab225715)Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for Cleaved PARP1 using ab225715 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cleaved PARP1 antibody [SP276] (ab225715)](https://www.abcam.com/ps/products/225/ab225715/Images/ab225715-286623-anti-cleaved-parp1-antibody-sp276-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cleaved PARP1 antibody [SP276] (ab225715)Formalin-fixed, paraffin-embedded human tonsil tissue stained for Cleaved PARP1 using ab225715 at 1/100 dilution in immunohistochemical analysis.
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![Western blot - Anti-Cleaved PARP1 antibody [SP276] (ab225715)](https://www.abcam.com/ps/products/225/ab225715/Images/ab225715-286622-anti-cleaved-parp1-antibody-sp276-western-blot.jpg)
Western blot - Anti-Cleaved PARP1 antibody [SP276] (ab225715)All lanes : Anti-Cleaved PARP1 antibody [SP276] (ab225715) at 1/100 dilution
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate treated with staurosporine
Lane 2 : Untreated NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Predicted band size: 113 kDa
Datasheets and documents
References
ab225715 has not yet been referenced specifically in any publications.
