Anti-Cleaved PARP1 antibody [Y34] (ab32561)
- Datasheet
- References (22)
- Protocols
Overview
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Product nameAnti-Cleaved PARP1 antibody [Y34]
See all Cleaved PARP1 primary antibodies -
DescriptionRabbit monoclonal [Y34] to Cleaved PARP1
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Host speciesRabbit
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SpecificityThis antibody is specific for p85 cleaved form of PARP1.
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Tested applicationsSuitable for: IHC-P, WB, Flow Cyt, IP, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide within Human Cleaved PARP1 aa 200-300. The exact sequence is proprietary. Residues following the cleavage of site.
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Positive control
- Jurkat cell lysate.
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General notes
A trial size is available to purchase for this antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityIgG fraction
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ClonalityMonoclonal
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Clone numberY34
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab32561 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. PubMed: 21931707 | |
| WB | 1/1000. Predicted molecular weight: 85 kDa. | |
| Flow Cyt | 1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IP | 1/50. | |
| ICC/IF | 1/500. |
Target
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FunctionInvolved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites.
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Sequence similaritiesContains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 2 PARP-type zinc fingers. -
Post-translational
modificationsPhosphorylated by PRKDC and TXK.
Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
S-nitrosylated, leading to inhibit transcription regulation activity. -
Cellular localizationNucleus. Nucleus, nucleolus. Localizes at sites of DNA damage.
- Information by UniProt
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Database links
- Entrez Gene: 142 Human
- Omim: 173870 Human
- SwissProt: P09874 Human
- Unigene: 177766 Human
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Alternative names
- ADP-ribosyltransferase diphtheria toxin-like 1 antibody
- ADPRT 1 antibody
- ADPRT antibody
see all
Images
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![Flow Cytometry - Anti-Cleaved PARP1 antibody [Y34] (ab32561)](https://www.abcam.com/ps/products/32/ab32561/Images/ab32561-280634-Untitledwbold2.jpg)
Flow Cytometry - Anti-Cleaved PARP1 antibody [Y34] (ab32561)Primary ab 1/50 dilution (0.5μg / Red). Secondary ab Goat anti rabbit IgG (FITC). Secondary ab concentration 1/150 dilution. Cell line Jurkat (human acute T cell leukemia) treated with (Right) or without (Left) 4μM Camptothecin for 5h. Fixative 4% paraformaldehyde. Datasheet comment Flow cytometric analysis of apoptotic and non-apoptotic Jurkat cells using anti-cleaved PARP1 RabMAb (ab32561). Jurkat cells were either left untreated (A) or treated with camptothecin (4 uM, 5 hr) to induce apoptosis (B). Cells were fixed and permeabilized, and then stained with anti-cleaved PARP1. The results indicate that 43% of cells were positive for cleaved PARP1 (B, M2) after treatment, compared to 9% positive without treatment (A, M2).
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![Western blot - Anti-Cleaved PARP1 antibody [Y34] (ab32561)](https://www.abcam.com/ps/products/32/ab32561/Images/ab32561-278220-ab32561KOWB.jpg)
Western blot - Anti-Cleaved PARP1 antibody [Y34] (ab32561)Lane 1: Wild type HAP1 (untreated) whole cell lysate (20 µg)
Lane 2: PARP1 (untreated) knockout HAP1 (untreated) whole cell lysate (20 µg)
Lane 3: HeLa (untreated) whole cell lysate (20 µg)
Lane 4: HAP1 (staurosporin treated, 1 u M, 4 hr) whole cell lysate (20 µg)
Lane 5: PARP1 (staurosporin treated, 1 uM, 4 hr) knockout HAP1 whole cell lysate (20 µg)
Lane 6: HeLa (staurosporin treated, 1 uM, 4 hr) whole cell lysate (20 µg)Lanes 1 - 6: Merged signal (red and green). Green - ab32561 observed at 100 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32561 was shown to specifically react with PARP1 (untreated) when PARP1 (untreated) knockout samples were used. Wild-type and PARP1 (untreated) knockout samples were subjected to SDS-PAGE. Ab32561 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-Cleaved PARP1 antibody [Y34] (ab32561)](https://www.abcam.com/ps/products/32/ab32561/Images/ab32561-237748-ab32561iccif.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cleaved PARP1 antibody [Y34] (ab32561)This image is courtesy of an anonymous Abreviewab32561 staining Cleaved PARP1 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. ab150081, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Counterstained with DAPI.
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![Western blot - Anti-Cleaved PARP1 antibody [Y34] (ab32561)](https://www.abcam.com/ps/products/32/ab32561/Images/ab32561.jpg)
Western blot - Anti-Cleaved PARP1 antibody [Y34] (ab32561)All lanes : Anti-Cleaved PARP1 antibody [Y34] (ab32561) at 1/1000 dilution
Lane 1 : Un-treated Jurkat cell lysate.
Lane 2 : Jurkat cell lysate treated with Camptothecin.
Predicted band size: 85 kDa
Observed band size: 85 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cleaved PARP1 antibody [Y34] (ab32561)](https://www.abcam.com/ps/products/32/ab32561/Images/Cleaved-PARP-Primary-antibodies-ab32561-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cleaved PARP1 antibody [Y34] (ab32561)Image from Wang L et al., PLoS One. 2011;6(9):e24405. Epub 2011 Sep 9. Fig 6.; doi:10.1371/journal.pone.0024405; September 9, 2011, PLoS ONE 6(9): e24405.Immunohistochemical analysis of OVCAR-3 tumour xenografts in nude mice, staining cleaved PARP1 with ab32561.
Antigen retrieval was performed via heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100) overnight at 4°C. A biotinyated anti-rabbit IgG (1/150) was used as the secondary antibody and staining was detected using DAB.
References
This product has been referenced in:
- Yang L et al. Sodium selenite induces apoptosis and inhibits autophagy in human synovial sarcoma cell line SW982 in vitro. Mol Med Rep 17:6560-6568 (2018). Read more (PubMed: 29512717) »
- Li JW et al. Six2 is negatively correlated with good prognosis and decreases 5-FU sensitivity via suppressing E-cadherin expression in hepatocellular carcinoma cells. Biomed Pharmacother 104:204-210 (2018). WB ; Human . Read more (PubMed: 29772441) »
