Immunocytochemistry/ Immunofluorescence abreview for Anti-Cleaved PARP1 antibody [Y34]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton-X100 in PBS
Specification
HeLa
Fixative
Formaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Dilution
1/500

Secondary antibody

Dilution
1/200

Additional data

Additional Notes
Asynchronous cells were treated with Staurosporine (1uM for 2 hours) to induce apoptosis. Cells were paraformaldehyde-fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. This antibody produces a strong, nuclear-enriched, focal staining pattern in cells exhibiting hallmarks of apoptosis. MeOH-fixed samples were also evaluated and gave a similar strong staining pattern.

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Submitted Nov 05 2014

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