Key features and details
- Rabbit polyclonal to CLEC10A
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-CLEC10A antibody
See all CLEC10A primary antibodies
DescriptionRabbit polyclonal to CLEC10A
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat
Recombinant fragment (His-T7-tag) corresponding to Mouse CLEC10A aa 69-273. Two N-terminal tags, expressed in E.coli.
RATLDNTTSKIKAEFQSLDSRADSFEKGISSLKVDVEDHRQELQAGRDLS QKVTSLESTVEKREQALKTDLSDLTDHVQQLRKDLKALTCQLANLKNNGS EVACCPLHWTEHEGSCYWFSESEKSWPEADKYCRLENSHLVVVNSLEEQN FLQNRLANVVSWIGLTDQNGPWRWVDGTDFEKGFKNWAPLQPDNWFGHGL GGGED
Database link: P49300
- WB: Recombinant mouse CLEC10A; Mouse lung, testis and cerebellum tissue lysate; Rat testis tissue lysate. IHC-P: Mouse liver and testis tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 0.05% Proclin 300, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232683 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 35 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionProbable role in regulating adaptive and innate immune responses. Binds in a calcium-dependent manner to terminal galactose and N-acetylgalactosamine units, linked to serine or threonine. These sugar moieties are known as Tn-Ag and are expressed in a variety of carcinoma cells.
Sequence similaritiesContains 1 C-type lectin domain.
- Information by UniProt
- C type (calcium dependent carbohydrate recognition domain) lectin superfamily member 13 (macrophage derived) antibody
- C type (calcium dependent carbohydrate recognition domain) lectin superfamily member 14 (macrophage derived) antibody
- C type lectin domain family 10, member A antibody
Formalin-fixed, paraffin-embedded mouse liver tissue stained for CLEC10A using ab232683 at 10μg/ml in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded mouse testis tissue stained for CLEC10A using ab232683 at 10μg/ml in immunohistochemical analysis.
Anti-CLEC10A antibody (ab232683) at 2 µg/ml + Recombinant mouse CLEC10A protein
HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution
Predicted band size: 35 kDa
All lanes : Anti-CLEC10A antibody (ab232683) at 2 µg/ml
Lane 1 : Mouse lung tissue lysate
Lane 2 : Mouse testis tissue lysate
Lane 3 : Mouse cerebellum tissue lysate
Lane 4 : Rat testis tissue lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution
Predicted band size: 35 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232683 has not yet been referenced specifically in any publications.