Anti-CLEC12A antibody - C-terminal (ab193941)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-CLEC12A antibody - C-terminal
See all CLEC12A primary antibodies -
Description
Goat polyclonal to CLEC12A - C-terminal -
Host species
Goat -
Specificity
ab193941 is expected to recognize all reported isoforms (NP_612210.4; NP_963917.2; NP_001193939.1). -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human CLEC12A aa 253-264 (C terminal) (Cysteine residue). NP_612210.4; NP_963917.2; NP_001193939.1.
Sequence:NPVQLGSTYFRE
Database link: Q5QGZ9 -
Positive control
- U937 cell line lysate.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab193941 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab193941 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 30 kDa). |
Target
-
Function
Cell surface receptor that modulates signaling cascades and mediates tyrosine phosphorylation of target MAP kinases. -
Tissue specificity
Detected in normal myeloid cells and in acute myeloid leukemia cells. Detected in neutrophils, eosinophils, monocytes and dendritic cells. Detected in spleen macrophage-rich red pulp and in lymph node (at protein level). Detected in peripheral blood leukocytes, dendritic cells, bone marrow, monocytes, mononuclear leukocytes and macrophages. -
Sequence similarities
Contains 1 C-type lectin domain. -
Domain
Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
Post-translational
modificationsHighly N-glycosylated. Glycosylation varies between cell types. -
Cellular localization
Cell membrane. Ligand binding leads to internalization. - Information by UniProt
-
Database links
- Entrez Gene: 160364 Human
- Omim: 612088 Human
- SwissProt: Q5QGZ9 Human
- Unigene: 190519 Human
-
Alternative names
- C-type lectin domain family 12 member A antibody
- C-type lectin-like molecule 1 antibody
- CL12A_HUMAN antibody
see all
Images
-
Western blot - Anti-CLEC12A antibody - C-terminal (ab193941)Anti-CLEC12A antibody - C-terminal (ab193941) at 0.3 µg + U937 cell line lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 30 kDa
Exposure time: 1 hour
Protocols
Datasheets and documents
References
ab193941 has not yet been referenced specifically in any publications.
