Anti-CLEC12A antibody - C-terminal (ab193941)
Key features and details
- Goat polyclonal to CLEC12A - C-terminal
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CLEC12A antibody - C-terminal
See all CLEC12A primary antibodies -
Description
Goat polyclonal to CLEC12A - C-terminal -
Host species
Goat -
Specificity
ab193941 is expected to recognize all reported isoforms (NP_612210.4; NP_963917.2; NP_001193939.1). -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human CLEC12A aa 253-264 (C terminal) (Cysteine residue). NP_612210.4; NP_963917.2; NP_001193939.1.
Sequence:NPVQLGSTYFRE
Database link: Q5QGZ9 -
Positive control
- U937 cell line lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab193941 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab193941 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 30 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Cell surface receptor that modulates signaling cascades and mediates tyrosine phosphorylation of target MAP kinases. -
Tissue specificity
Detected in normal myeloid cells and in acute myeloid leukemia cells. Detected in neutrophils, eosinophils, monocytes and dendritic cells. Detected in spleen macrophage-rich red pulp and in lymph node (at protein level). Detected in peripheral blood leukocytes, dendritic cells, bone marrow, monocytes, mononuclear leukocytes and macrophages. -
Sequence similarities
Contains 1 C-type lectin domain. -
Domain
Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
Post-translational
modificationsHighly N-glycosylated. Glycosylation varies between cell types. -
Cellular localization
Cell membrane. Ligand binding leads to internalization. - Information by UniProt
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Database links
- Entrez Gene: 160364 Human
- Omim: 612088 Human
- SwissProt: Q5QGZ9 Human
- Unigene: 190519 Human
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Alternative names
- C-type lectin domain family 12 member A antibody
- C-type lectin-like molecule 1 antibody
- CL12A_HUMAN antibody
see all
Images
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Western blot - Anti-CLEC12A antibody - C-terminal (ab193941)Anti-CLEC12A antibody - C-terminal (ab193941) at 0.3 µg + U937 cell line lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 30 kDa
Exposure time: 1 hour
Datasheets and documents
References (0)
ab193941 has not yet been referenced specifically in any publications.
