• Product name
    Anti-CLEC4E antibody [AT16E3]
    See all CLEC4E primary antibodies
  • Description
    Mouse monoclonal [AT16E3] to CLEC4E
  • Host species
  • Tested applications
    Suitable for: IHC-P, WB, ELISA, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment purified from E. coli, corresponding to amino acids 41-219 of Human CLEC4E (NP_055173)

  • Positive control
    • HeLa cells and HeLa cell lysate
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
  • Clone number
  • Isotype
  • Light chain type
  • Research areas


Our Abpromise guarantee covers the use of ab100846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/250 - 1/500. Predicted molecular weight: 25 kDa.
ELISA Use at an assay dependent concentration.
ICC/IF 1/250 - 1/500.
Flow Cyt Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.



  • Function
    C-type lectin that functions as cell-surface receptor for a wide variety of ligands such as damaged cells, fungi and mycobacteria. Plays a role in the recognition of pathogenic fungi, such as Candida albicans. The detection of mycobacteria is via trehalose 6,6'-dimycolate (TDM), a cell wall glycolipid. Specifically recognizes alpha-mannose residues on pathogenic fungi of the genus Malassezia. Recognizes also SAP130, a nuclear protein, that is released by dead or dying cells. Transduces signals through an ITAM-containing adapter protein, Fc receptor gamma chain /FCER1G. Induces secretion of inflammatory cytokines through a pathway that depends on SYK, CARD9 and NF-kappa-B.
  • Sequence similarities
    Contains 1 C-type lectin domain.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • C type lectin domain family 4 member E antibody
    • C type lectin superfamily member 9 antibody
    • C-type (calcium dependent carbohydrate recognition domain) lectin superfamily member 9 antibody
    • C-type lectin domain family 4 member E antibody
    • C-type lectin superfamily member 9 antibody
    • CLC4E_HUMAN antibody
    • CLEC 4E antibody
    • Clec4e antibody
    • CLECSF9 antibody
    • Macrophage inducible C type lectin antibody
    • Macrophage-inducible C-type lectin antibody
    • MINCLE antibody
    see all


  • Anti-CLEC4E antibody [AT16E3] (ab100846) at 1/1000 dilution + HeLa Cell Lysate at 35 µg

    goat anti-mouse conjugated to HRP

    Developed using the ECL technique.

    Predicted band size: 25 kDa

  • Immunofluorescence of Human HeLa cells stained with ab100846 (1/500) with Texas Red (Red). Nucleus was stained by Hoechst 33342 (Blue).
  • ab100846 staining CLEC4E in colorectal cancer tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were subjected to antigen retrieval in 0.1M citric acid. The primary antibody was diluted 1/50 and incubated with the sample for 2 hours at room temperature. Antibody was detected by DAB staining.
  • Overlay histogram showing HeLa cells stained with ab100846 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab100846, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91633, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.


ab100846 has not yet been referenced specifically in any publications.

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