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  1. Link

    clec9a-antibody-1f6-ab104903.pdf

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Stem Cells Hematopoietic Progenitors Surface Molecules
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Anti-CLEC9A antibody [1F6] (ab104903)

  • Datasheet
  • SDS
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Promotion Information

Abpromise

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Find out more.

Key features and details

  • Rat monoclonal [1F6] to CLEC9A
  • Suitable for: ICC/IF, Flow Cyt, WB
  • Reacts with: Mouse
  • Isotype: IgG1

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-CLEC9A antibody [EPR22324] (ab223188)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-CLEC9A antibody [1F6]
    See all CLEC9A primary antibodies
  • Description

    Rat monoclonal [1F6] to CLEC9A
  • Host species

    Rat
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Tissue, cells or virus corresponding to CLEC9A. RBL-2H3 cells expressing Mouse CLEC9A fused to an HA epitope.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Growth Conditions: RPMI + 10% FCS + Pen + Strep + Glutamine + 2-ME.
  • Clonality

    Monoclonal
  • Clone number

    1F6
  • Myeloma

    Y3/Ag1.2.3
  • Isotype

    IgG1
  • Research areas

    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules
    • Immunology
    • Innate Immunity
    • Myeloid Cells

Associated products

  • Compatible Secondaries

    • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
    • Goat Anti-Rat IgG H&L (HRP) (ab205720)
  • Isotype control

    • Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (ab18412)
  • Recombinant Protein

    • Recombinant Human CLEC9A protein (ab132795)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab104903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB
Use at an assay dependent concentration.
Notes
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB
Use at an assay dependent concentration.

Target

  • Function

    Functions as an endocytic receptor on a small subset of myeloid cells specialized for the uptake and processing of material from dead cells. Recognizes filamentous form of actin in association with particular actin-binding domains of cytoskeletal proteins, including spectrin, exposed when cell membranes are damaged, and mediate the cross-presentation of dead-cell associated antigens in a Syk-dependent manner.
  • Tissue specificity

    In peripheral blood highly restricted on the surface of BDCA31(+) dendritic cells and on a small subset of CD14(+) and CD16(-) monocytes.
  • Sequence similarities

    Contains 1 C-type lectin domain.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession Q6UXN8 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 232414 Mouse
    • SwissProt: Q8BRU4 Mouse
    • Unigene: 391518 Mouse
    • Alternative names

      • C type lectin domain family 9, member A antibody
      • C-type lectin domain family 9 member A antibody
      • CLC9A_HUMAN antibody
      • CLEC9A antibody
      • DNGR1 antibody
      • HEEE9341 antibody
      • PRO34046 antibody
      • UNQ9341 antibody
      see all

    Protocols

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab104903? Please let us know so that we can cite the reference in this datasheet.

    ab104903 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CLEC9A antibody [1F6]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (spleen)
    Specification
    spleen
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: rt°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 15 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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