Anti-CLEC9A antibody [8F9] (ab104910)
Key features and details
- Mouse monoclonal [8F9] to CLEC9A
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-CLEC9A antibody [8F9]
See all CLEC9A primary antibodies -
Description
Mouse monoclonal [8F9] to CLEC9A -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
RBL-2H3 cells expressing Human CLEC9A fused to an HA epitope
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Positive control
- ICC/IF: MCF7 cells.
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General notes
Growth Conditions: RPMI + 10% FCS + Pen + Strep + Glutamine + 2-ME.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
8F9 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab104910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. Block Fc receptor prior to staining to reduce background.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|
|
| ICC/IF | Use a concentration of 5 µg/ml. |
Target
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Function
Functions as an endocytic receptor on a small subset of myeloid cells specialized for the uptake and processing of material from dead cells. Recognizes filamentous form of actin in association with particular actin-binding domains of cytoskeletal proteins, including spectrin, exposed when cell membranes are damaged, and mediate the cross-presentation of dead-cell associated antigens in a Syk-dependent manner. -
Tissue specificity
In peripheral blood highly restricted on the surface of BDCA31(+) dendritic cells and on a small subset of CD14(+) and CD16(-) monocytes. -
Sequence similarities
Contains 1 C-type lectin domain. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 283420 Human
- Omim: 612252 Human
- SwissProt: Q6UXN8 Human
- Unigene: 531189 Human
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Alternative names
- C type lectin domain family 9, member A antibody
- C-type lectin domain family 9 member A antibody
- CLC9A_HUMAN antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-CLEC9A antibody [8F9] (ab104910)](https://www.abcam.com/ps/products/104/ab104910/Images/ab104910-196278-anti-clec9a-antibody-8f9-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CLEC9A antibody [8F9] (ab104910)ICC/IF image of ab104910 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104910, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Datasheets and documents
References
ab104910 has been referenced in 2 publications.
- Canavan M et al. Enriched Cd141+ DCs in the joint are transcriptionally distinct, activated, and contribute to joint pathogenesis. JCI Insight 3:N/A (2018). PubMed: 30518680
- Sancho D et al. Tumor therapy in mice via antigen targeting to a novel, DC-restricted C-type lectin. J Clin Invest 118:2098-110 (2008). Human . PubMed: 18497879
