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  1. Link

    clec9a-antibody-8f9-ab104910.pdf

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Stem Cells Hematopoietic Progenitors Surface Molecules
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Anti-CLEC9A antibody [8F9] (ab104910)

  • Datasheet
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Immunocytochemistry/ Immunofluorescence - Anti-CLEC9A antibody [8F9] (ab104910)

    Key features and details

    • Mouse monoclonal [8F9] to CLEC9A
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Isotype: IgG2a

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    Recombinant Human CLEC9A protein (ab132795)
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    Anti-CD15 antibody [FUT4/815] - BSA and Azide free (ab212396)

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    Overview

    • Product name

      Anti-CLEC9A antibody [8F9]
      See all CLEC9A primary antibodies
    • Description

      Mouse monoclonal [8F9] to CLEC9A
    • Host species

      Mouse
    • Tested applications

      Suitable for: ICC/IFmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Tissue, cells or virus corresponding to CLEC9A. RBL-2H3 cells expressing Human CLEC9A fused to an HA epitope

    • Positive control

      • ICC/IF: MCF7 cells.
    • General notes

      Growth Conditions: RPMI + 10% FCS + Pen + Strep + Glutamine + 2-ME.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Monoclonal
    • Clone number

      8F9
    • Myeloma

      Sp2/0-Ag14
    • Isotype

      IgG2a
    • Research areas

      • Stem Cells
      • Hematopoietic Progenitors
      • Surface Molecules
      • Immunology
      • Innate Immunity
      • Myeloid Cells

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Conjugation kits

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • APC Conjugation Kit - Lightning-Link® (ab201807)
    • Isotype control

      • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
    • Recombinant Protein

      • Recombinant Human CLEC9A protein (ab132795)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab104910 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF
    Use a concentration of 5 µg/ml.
    Notes
    ICC/IF
    Use a concentration of 5 µg/ml.

    Target

    • Function

      Functions as an endocytic receptor on a small subset of myeloid cells specialized for the uptake and processing of material from dead cells. Recognizes filamentous form of actin in association with particular actin-binding domains of cytoskeletal proteins, including spectrin, exposed when cell membranes are damaged, and mediate the cross-presentation of dead-cell associated antigens in a Syk-dependent manner.
    • Tissue specificity

      In peripheral blood highly restricted on the surface of BDCA31(+) dendritic cells and on a small subset of CD14(+) and CD16(-) monocytes.
    • Sequence similarities

      Contains 1 C-type lectin domain.
    • Post-translational
      modifications

      N-glycosylated.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession Q6UXN8 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 283420 Human
      • Omim: 612252 Human
      • SwissProt: Q6UXN8 Human
      • Unigene: 531189 Human
      • Alternative names

        • C type lectin domain family 9, member A antibody
        • C-type lectin domain family 9 member A antibody
        • CLC9A_HUMAN antibody
        • CLEC9A antibody
        • DNGR1 antibody
        • HEEE9341 antibody
        • PRO34046 antibody
        • UNQ9341 antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-CLEC9A antibody [8F9] (ab104910)
        Immunocytochemistry/ Immunofluorescence - Anti-CLEC9A antibody [8F9] (ab104910)

        ICC/IF image of ab104910 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104910, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

      Protocols

      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (3)

      Publishing research using ab104910? Please let us know so that we can cite the reference in this datasheet.

      ab104910 has been referenced in 3 publications.

      • Ren X  et al. Reconstruction of cell spatial organization from single-cell RNA sequencing data based on ligand-receptor mediated self-assembly. Cell Res 30:763-778 (2020). PubMed: 32541867
      • Canavan M  et al. Enriched Cd141+ DCs in the joint are transcriptionally distinct, activated, and contribute to joint pathogenesis. JCI Insight 3:N/A (2018). PubMed: 30518680
      • Sancho D  et al. Tumor therapy in mice via antigen targeting to a novel, DC-restricted C-type lectin. J Clin Invest 118:2098-110 (2008). Human . PubMed: 18497879

      Customer reviews and Q&As

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