Recombinant Anti-CLEC9A antibody [EPR22324] (ab223188)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22324] to CLEC9A
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CLEC9A antibody [EPR22324]
See all CLEC9A primary antibodies -
Description
Rabbit monoclonal [EPR22324] to CLEC9A -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, Flow Cytmore details
Unsuitable for: ICC/IF,IP or WB -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human tonsil, thymoma and gastric cancer tissues. Flow Cyt: Human peripheral blood mononuclear cell (PBMC) and HEK-293T transfected with GFP-tagged CLEC9A overexpression construct cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22324 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223188 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Flow Cyt |
1/50.
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Notes |
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IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt
1/50. |
Target
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Function
Functions as an endocytic receptor on a small subset of myeloid cells specialized for the uptake and processing of material from dead cells. Recognizes filamentous form of actin in association with particular actin-binding domains of cytoskeletal proteins, including spectrin, exposed when cell membranes are damaged, and mediate the cross-presentation of dead-cell associated antigens in a Syk-dependent manner. -
Tissue specificity
In peripheral blood highly restricted on the surface of BDCA31(+) dendritic cells and on a small subset of CD14(+) and CD16(-) monocytes. -
Sequence similarities
Contains 1 C-type lectin domain. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 283420 Human
- Omim: 612252 Human
- SwissProt: Q6UXN8 Human
- Unigene: 531189 Human
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Alternative names
- C type lectin domain family 9, member A antibody
- C-type lectin domain family 9 member A antibody
- CLC9A_HUMAN antibody
see all
Images
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Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with GFP-tagged CLEC9A overexpression construct, labeling CLEC9A with ab223188 at 1/500 dilution (Right) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) was used as the secondary antibody at 1/2000 dilution. Gated on viable cells.
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Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labeling CLEC9A with ab223188 at 1/50 dilution. Cells were stained with Rabbit monoclonal IgG (ab172730) (Left) or ab223188 (Right). Then stained with Alexa Fluor® 647-conjugated anti-CD3, PE conjugated anti-HLA-DR and BV421 conjugated anti-CD141. Data shown was firstly gated out lymphocytes, then gated on viable CD3(-) HLA-DR (+) APC cells.
CLEC9A is selectively expressed on CD141+DC cells (Q2, ~0.05% in PBMC) and expression pattern was described in literature. (PMID:20479116).
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Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling CLEC9A with ab223188 at 1/1000 dilution, followed Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on dendritic cells (arrows) of human gastric cancer (PMID: 18408006; PMID: 20479116) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human thyoma tissue labeling CLEC9A with ab223188 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on dendritic cells of human thymoma (PMID: 18408006; PMID: 20479116) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CLEC9A with ab223188 at 1/1000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB Ready to use (ab209101). Positive staining on dendritic cells of human tonsil (PMID: 18408006; PMID: 20479116) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB Ready to use (ab209101).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (5)
ab223188 has been referenced in 5 publications.
- Xiong W et al. Smad4 Deficiency Promotes Pancreatic Cancer Immunogenicity by Activating the Cancer-Autonomous DNA-Sensing Signaling Axis. Adv Sci (Weinh) 9:e2103029 (2022). PubMed: 35064757
- Anadon CM et al. Ovarian cancer immunogenicity is governed by a narrow subset of progenitor tissue-resident memory T cells. Cancer Cell 40:545-557.e13 (2022). PubMed: 35427494
- Papadas A et al. Stromal remodeling regulates dendritic cell abundance and activity in the tumor microenvironment. Cell Rep 40:111201 (2022). PubMed: 35977482
- Chevrier S et al. A distinct innate immune signature marks progression from mild to severe COVID-19. Cell Rep Med 2:100166 (2021). PubMed: 33521697
- Moysi E et al. In Situ Characterization of Human Lymphoid Tissue Immune Cells by Multispectral Confocal Imaging and Quantitative Image Analysis; Implications for HIV Reservoir Characterization. Front Immunol 12:683396 (2021). PubMed: 34177929