Recombinant
RabMAb

Recombinant Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (ab245121)

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Overview

  • Product name

    Anti-CLEC9A antibody [EPR22324] - BSA and Azide free
    See all CLEC9A primary antibodies

  • Description

    Rabbit monoclonal [EPR22324] to CLEC9A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF,IP or WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human CLEC9A aa 150 to the C-terminus. The exact sequence is proprietary.
    Database link: Q6UXN8

  • Positive control

    • IHC-P: Human tonsil, thymoma and gastric cancer tissues. Flow Cyt: Human peripheral blood mononuclear cell (PBMC) and HEK-293T transfected with GFP-tagged CLEC9A overexpression construct cells.

  • General notes

    Ab245121 is the carrier-free version of ab223188. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab245121 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab245121 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC/IF,IP or WB.

    • Target

    • Function

      Functions as an endocytic receptor on a small subset of myeloid cells specialized for the uptake and processing of material from dead cells. Recognizes filamentous form of actin in association with particular actin-binding domains of cytoskeletal proteins, including spectrin, exposed when cell membranes are damaged, and mediate the cross-presentation of dead-cell associated antigens in a Syk-dependent manner.

    • Tissue specificity

      In peripheral blood highly restricted on the surface of BDCA31(+) dendritic cells and on a small subset of CD14(+) and CD16(-) monocytes.

    • Sequence similarities

      Contains 1 C-type lectin domain.

    • Post-translational
      modifications

      N-glycosylated.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • C type lectin domain family 9, member A antibody
        • C-type lectin domain family 9 member A antibody
        • CLC9A_HUMAN antibody
        • CLEC9A antibody
        • DNGR1 antibody
        • HEEE9341 antibody
        • PRO34046 antibody
        • UNQ9341 antibody
        see all

      Images

      • Flow Cytometry - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (ab245121)
        Flow Cytometry - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (ab245121)

        Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with GFP-tagged CLEC9A overexpression construct, labeling CLEC9A with ab223188 at 1/500 dilution (Right) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) was used as the secondary antibody at 1/2000 dilution. Gated on viable cells.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223188).

      • Flow Cytometry - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (ab245121)
        Flow Cytometry - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (ab245121)

        Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labeling CLEC9A with ab223188 at 1/50 dilution. Cells were stained with Rabbit monoclonal IgG (ab172730) (Left) or ab223188 (Right). Then stained with Alexa Fluor® 647-conjugated anti-CD3, PE conjugated anti-HLA-DR and BV421 conjugated anti-CD141. Data shown was firstly gated out lymphocytes, then gated on viable CD3(-) HLA-DR (+) APC cells.

        CLEC9A is selectively expressed on CD141+DC cells (Q2, ~0.05% in PBMC) and expression pattern was described in literature. (PMID:20479116).

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223188).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (ab245121)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (ab245121)

        Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling CLEC9A with ab223188 at 1/1000 dilution, followed Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on dendritic cells (arrows) of human gastric cancer (PMID: 18408006; PMID: 20479116) is observed. Counterstained with hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223188).

        Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (ab245121)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (ab245121)

        Immunohistochemical analysis of paraffin-embedded human thyoma tissue labeling CLEC9A with ab223188 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on dendritic cells of human thymoma (PMID: 18408006; PMID: 20479116) is observed. Counterstained with hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223188).

        Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (ab245121)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (ab245121)

        Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CLEC9A with ab223188 at 1/1000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB Ready to use (ab209101). Positive staining on dendritic cells of human tonsil (PMID: 18408006; PMID: 20479116) is observed. Counterstained with hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB Ready to use (ab209101).

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223188).

        Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      References

      ab245121 has not yet been referenced specifically in any publications.

      Publishing research using ab245121? Please let us know so that we can cite the reference in this datasheet.

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