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RecombinantRabMAb

Recombinant Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (ab245121)

Reviews (1) Submit a question
Flow Cytometry - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (ab245121)
  • Flow Cytometry - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (ab245121)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (ab245121)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (ab245121)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (ab245121)
  • Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (ab245121)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22324] to CLEC9A - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-CLEC9A antibody [EPR22324] - BSA and Azide free
    See all CLEC9A primary antibodies

  • Description

    Rabbit monoclonal [EPR22324] to CLEC9A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF,IP or WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human tonsil, thymoma and gastric cancer tissues. Flow Cyt: Human peripheral blood mononuclear cell (PBMC) and HEK-293T transfected with GFP-tagged CLEC9A overexpression construct cells.

  • General notes

    ab245121 is the carrier-free version of ab223188.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab245121 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt
Use at an assay dependent concentration.
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt
Use at an assay dependent concentration.
Application notes
Is unsuitable for ICC/IF,IP or WB.

Target

  • Function

    Functions as an endocytic receptor on a small subset of myeloid cells specialized for the uptake and processing of material from dead cells. Recognizes filamentous form of actin in association with particular actin-binding domains of cytoskeletal proteins, including spectrin, exposed when cell membranes are damaged, and mediate the cross-presentation of dead-cell associated antigens in a Syk-dependent manner.

  • Tissue specificity

    In peripheral blood highly restricted on the surface of BDCA31(+) dendritic cells and on a small subset of CD14(+) and CD16(-) monocytes.

  • Sequence similarities

    Contains 1 C-type lectin domain.

  • Post-translational
    modifications

    N-glycosylated.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • C type lectin domain family 9, member A antibody
      • C-type lectin domain family 9 member A antibody
      • CLC9A_HUMAN antibody
      • CLEC9A antibody
      • DNGR1 antibody
      • HEEE9341 antibody
      • PRO34046 antibody
      • UNQ9341 antibody
      see all

    Images

    • Flow Cytometry - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (ab245121)
      Flow Cytometry - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (ab245121)

      Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with GFP-tagged CLEC9A overexpression construct, labeling CLEC9A with ab223188 at 1/500 dilution (Right) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) was used as the secondary antibody at 1/2000 dilution. Gated on viable cells.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223188).

    • Flow Cytometry - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (ab245121)
      Flow Cytometry - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (ab245121)

      Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labeling CLEC9A with ab223188 at 1/50 dilution. Cells were stained with Rabbit monoclonal IgG (ab172730) (Left) or ab223188 (Right). Then stained with Alexa Fluor® 647-conjugated anti-CD3, PE conjugated anti-HLA-DR and BV421 conjugated anti-CD141. Data shown was firstly gated out lymphocytes, then gated on viable CD3(-) HLA-DR (+) APC cells.

      CLEC9A is selectively expressed on CD141+DC cells (Q2, ~0.05% in PBMC) and expression pattern was described in literature. (PMID:20479116).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223188).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (ab245121)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (ab245121)

      Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling CLEC9A with ab223188 at 1/1000 dilution, followed Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on dendritic cells (arrows) of human gastric cancer (PMID: 18408006; PMID: 20479116) is observed. Counterstained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223188).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (ab245121)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (ab245121)

      Immunohistochemical analysis of paraffin-embedded human thyoma tissue labeling CLEC9A with ab223188 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on dendritic cells of human thymoma (PMID: 18408006; PMID: 20479116) is observed. Counterstained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223188).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (ab245121)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (ab245121)

      Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CLEC9A with ab223188 at 1/1000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB Ready to use (ab209101). Positive staining on dendritic cells of human tonsil (PMID: 18408006; PMID: 20479116) is observed. Counterstained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB Ready to use (ab209101).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223188).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (ab245121)
      Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (ab245121)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab245121? Please let us know so that we can cite the reference in this datasheet.

    ab245121 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Immunohistochemistry (Frozen sections) abreview for Anti-CLEC9A antibody [EPR22324] - BSA and Azide free

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Human Tonsil)
    Permeabilization
    No
    Specification
    Human Tonsil
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Acetone
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Sep 30 2021

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