Overview

  • Product name

  • Description

    Goat polyclonal to CLIC1
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Pig
  • Immunogen

    Synthetic peptide corresponding to Human CLIC1 aa 45-57 (Cysteine residue).
    Sequence:

    C-TVDTKRRTETVQK


    Database link: O00299

  • Positive control

    • WB: U937 (human histiocytic lymphoma cell line) cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab223216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml.

Target

  • Function

    Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Involved in regulation of the cell cycle.
  • Tissue specificity

    Expression is prominent in heart, placenta, liver, kidney and pancreas.
  • Sequence similarities

    Belongs to the chloride channel CLIC family.
    Contains 1 GST C-terminal domain.
  • Domain

    Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.
  • Post-translational
    modifications

    Hydrogen peroxide treatment causes a conformation change, leading to dimerization and formation of an intramolecular disulfide bond between Cys-24 and Cys-59.
  • Cellular localization

    Nucleus. Nucleus membrane. Cytoplasm. Cell membrane. Mostly in the nucleus including in the nuclear membrane. Small amount in the cytoplasm and the plasma membrane. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • Chloride channel ABP antibody
    • Chloride intracellular channel 1 antibody
    • Chloride intracellular channel protein 1 antibody
    • CLIC 1 antibody
    • CLIC1 antibody
    • CLIC1_HUMAN antibody
    • G6 antibody
    • hRNCC antibody
    • NCC 27 antibody
    • NCC27 antibody
    • Nuclear chloride ion channel 27 antibody
    • Nuclear chloride ion channel protein antibody
    • p64CLCP antibody
    • Regulatory nuclear chloride ion channel protein antibody
    • RNCC protein antibody
    see all

Images

  • Anti-CLIC1 antibody (ab223216) at 2 µg/ml + U937 (human histiocytic lymphoma cell line) cell lysate in RIPA buffer at 35 µg


    Primary incubation: 1 hour

References

ab223216 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223216.
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