Key features and details
- Rabbit polyclonal to CLIC1
- Suitable for: ELISA, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CLIC1 antibody
See all CLIC1 primary antibodies
DescriptionRabbit polyclonal to CLIC1
Specificityab28722 recognises Chloride intracellular channel protein 1(CLIC1)
Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Zebrafish
- Jurkat cell lysate, Human kidney tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab28722 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1.25 µg/ml. Predicted molecular weight: 27 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use at an assay dependent concentration.|
FunctionCan insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Involved in regulation of the cell cycle.
Tissue specificityExpression is prominent in heart, placenta, liver, kidney and pancreas.
Sequence similaritiesBelongs to the chloride channel CLIC family.
Contains 1 GST C-terminal domain.
DomainMembers of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.
modificationsHydrogen peroxide treatment causes a conformation change, leading to dimerization and formation of an intramolecular disulfide bond between Cys-24 and Cys-59.
Cellular localizationNucleus. Nucleus membrane. Cytoplasm. Cell membrane. Mostly in the nucleus including in the nuclear membrane. Small amount in the cytoplasm and the plasma membrane. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain.
- Information by UniProt
- Chloride channel ABP antibody
- Chloride intracellular channel 1 antibody
- Chloride intracellular channel protein 1 antibody
Anti-CLIC1 antibody (ab28722) at 1.25 µg/ml + Jurkat cell lysate
HRP conjugated anti-Rabbit IgG 1: 50,000 - 100,000
Predicted band size: 27 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Immunohistochemistry analysis of human kidney tissue labelling CLIC1 with ab28722.
ab28722 has been referenced in 2 publications.
- Zhou J et al. Proteomics-Based Identification and Analysis of Proteins Associated with Helicobacter pylori in Gastric Cancer. PLoS One 11:e0146521 (2016). WB . PubMed: 26745502
- Chen WQ et al. Variations of protein levels in human amniotic fluid stem cells CD117/2 over passages 5-25. J Proteome Res 8:5285-95 (2009). PubMed: 19791749