• Product name

    Anti-CLIC2 antibody [EPR6494]
    See all CLIC2 primary antibodies
  • Description

    Rabbit monoclonal [EPR6494] to CLIC2
  • Host species

  • Specificity

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
  • Tested applications

    Suitable for: ICC/IF, IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CLIC2 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: O15247

  • Positive control

    • Human fetal liver, Human heart and Human testis lysates; Human liver tissue; HepG2 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

  • Clone number

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab175230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100.
WB 1/1000 - 1/10000. Predicted molecular weight: 28 kDa.


  • Function

    Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Modulates the activity of RYR2 and inhibits calcium influx.
  • Tissue specificity

    Detected in adult brain, heart, liver, lung, spleen, stomach and testis. Expressed in fetal liver and adult skeletal muscle.
  • Sequence similarities

    Belongs to the chloride channel CLIC family.
    Contains 1 GST C-terminal domain.
  • Domain

    Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.
  • Cellular localization

    Cytoplasm. Membrane. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • Chloride intracellular channel 2 antibody
    • Chloride intracellular channel protein 2 antibody
    • CLIC 2b antibody
    • CLIC2 antibody
    • CLIC2_HUMAN antibody
    • CLIC2b antibody
    • MRXS32 antibody
    • OTTHUMP00000196471 antibody
    • XAP 121 antibody
    • XAP121 antibody
    see all


  • All lanes : Anti-CLIC2 antibody [EPR6494] (ab175230) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human heart lysate
    Lane 3 : Human testis lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 28 kDa

  • Western blot analysis on immunoprecipitation pellet from (1) fetal liver lysate or (2) 1X PBS (negative control) using ab175230, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Immunofluorescent analysis of HepG2 cells labeling CLIC2 with ab175230 at 1/100 dilution (red). DAPI nuclear staining (blue).

  • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CLIC2 with ab175230 at 1/50 dilution.


ab175230 has not yet been referenced specifically in any publications.

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