Overview

  • Product name
    Anti-CLIC2 antibody [EPR6494]
    See all CLIC2 primary antibodies
  • Description
    Rabbit monoclonal [EPR6494] to CLIC2
  • Host species
    Rabbit
  • Specificity
    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
  • Tested applications
    Suitable for: ICC/IF, IP, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CLIC2 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: O15247

  • Positive control
    • Human fetal liver, Human heart and Human testis lysates; Human liver tissue; HepG2 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR6494
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100.
WB 1/1000 - 1/10000. Predicted molecular weight: 28 kDa.

Target

  • Function
    Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Modulates the activity of RYR2 and inhibits calcium influx.
  • Tissue specificity
    Detected in adult brain, heart, liver, lung, spleen, stomach and testis. Expressed in fetal liver and adult skeletal muscle.
  • Sequence similarities
    Belongs to the chloride channel CLIC family.
    Contains 1 GST C-terminal domain.
  • Domain
    Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.
  • Cellular localization
    Cytoplasm. Membrane. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chloride intracellular channel 2 antibody
    • Chloride intracellular channel protein 2 antibody
    • CLIC 2b antibody
    • CLIC2 antibody
    • CLIC2_HUMAN antibody
    • CLIC2b antibody
    • MRXS32 antibody
    • OTTHUMP00000196471 antibody
    • XAP 121 antibody
    • XAP121 antibody
    see all

Images

  • All lanes : Anti-CLIC2 antibody [EPR6494] (ab175230) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human heart lysate
    Lane 3 : Human testis lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 28 kDa

  • Western blot analysis on immunoprecipitation pellet from (1) fetal liver lysate or (2) 1X PBS (negative control) using ab175230, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Immunofluorescent analysis of HepG2 cells labeling CLIC2 with ab175230 at 1/100 dilution (red). DAPI nuclear staining (blue).

  • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CLIC2 with ab175230 at 1/50 dilution.

References

ab175230 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab175230.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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