Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6494] to CLIC2
- Suitable for: ICC/IF, IP, IHC-P, WB
- Reacts with: Human
Product nameAnti-CLIC2 antibody [EPR6494]
See all CLIC2 primary antibodies
DescriptionRabbit monoclonal [EPR6494] to CLIC2
SpecificityMouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Tested applicationsSuitable for: ICC/IF, IP, IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human CLIC2 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: O15247
- Human fetal liver, Human heart and Human testis lysates; Human liver tissue; HepG2 cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab175230 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|IP||1/10 - 1/100.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 28 kDa.|
FunctionCan insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Modulates the activity of RYR2 and inhibits calcium influx.
Tissue specificityDetected in adult brain, heart, liver, lung, spleen, stomach and testis. Expressed in fetal liver and adult skeletal muscle.
Sequence similaritiesBelongs to the chloride channel CLIC family.
Contains 1 GST C-terminal domain.
DomainMembers of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.
Cellular localizationCytoplasm. Membrane. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain.
- Information by UniProt
- Chloride intracellular channel 2 antibody
- Chloride intracellular channel protein 2 antibody
- CLIC 2b antibody
All lanes : Anti-CLIC2 antibody [EPR6494] (ab175230) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human heart lysate
Lane 3 : Human testis lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 28 kDa
Western blot analysis on immunoprecipitation pellet from (1) fetal liver lysate or (2) 1X PBS (negative control) using ab175230, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Immunofluorescent analysis of HepG2 cells labeling CLIC2 with ab175230 at 1/100 dilution (red). DAPI nuclear staining (blue).
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CLIC2 with ab175230 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab175230 has been referenced in 1 publication.
- Liu G et al. Analysis of Differentially Expressed Genes in a Chinese Cohort of Esophageal Squamous Cell Carcinoma. J Cancer 11:3783-3793 (2020). PubMed: 32328183