Recombinant Anti-CLIC2 antibody [EPR6495] (ab126727)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6495] to CLIC2
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-CLIC2 antibody [EPR6495]
See all CLIC2 primary antibodies -
Description
Rabbit monoclonal [EPR6495] to CLIC2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Human fetal liver, Human heart, and Human testis lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 3.10 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6495 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126727 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
|
Notes |
---|
WB
1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). |
Target
-
Function
Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Modulates the activity of RYR2 and inhibits calcium influx. -
Tissue specificity
Detected in adult brain, heart, liver, lung, spleen, stomach and testis. Expressed in fetal liver and adult skeletal muscle. -
Sequence similarities
Belongs to the chloride channel CLIC family.
Contains 1 GST C-terminal domain. -
Domain
Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion. -
Cellular localization
Cytoplasm. Membrane. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain. - Information by UniProt
-
Database links
- Entrez Gene: 1193 Human
- Entrez Gene: 294141 Rat
- Omim: 300138 Human
- SwissProt: O15247 Human
- SwissProt: Q5M883 Rat
- Unigene: 655445 Human
- Unigene: 103254 Rat
-
Alternative names
- Chloride intracellular channel 2 antibody
- Chloride intracellular channel protein 2 antibody
- CLIC 2b antibody
see all
Images
-
All lanes : Anti-CLIC2 antibody [EPR6495] (ab126727) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human heart lysate
Lane 3 : Human testis lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 28 kDa
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab126727 has not yet been referenced specifically in any publications.