Product nameAnti-CLIC3 antibody [EPR8243(B)]
See all CLIC3 primary antibodies
DescriptionRabbit monoclonal [EPR8243(B)] to CLIC3
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human CLIC3 aa 50-150. The exact sequence is proprietary.
- JAR cell lysate; Rat lung tissue lysate; Human placenta tissue
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab128941 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevanceChloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 3 is a member of the p64 family and is predominantly localized in the nucleus and stimulates chloride ion channel activity. In addition, this protein may participate in cellular growth control, based on its association with ERK7, a member of the MAP kinase family.
Cellular localizationNuclear, cytoplasmic and Single pass membrane. Predominantly nuclear but also found in the cytoplasm. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain
- 2300003G24Rik antibody
- Chloride intracellular channel 3 antibody
- Chloride intracellular channel protein 3 antibody
All lanes : Anti-CLIC3 antibody [EPR8243(B)] (ab128941) at 1/1000 dilution
Lane 1 : JAR cell lysate
Lane 2 : Rat lung tissue lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDa
ab128941 at 1/50 staining CLIC3 in paraffin-embedded Human placenta tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab128941 has not yet been referenced specifically in any publications.