• Product name
  • Description
    Goat polyclonal to CLIC4
  • Host species
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog
  • Immunogen

    Synthetic peptide:


    , corresponding to N terminal amino acids 8-20 of Human CLIC4 (NP_039234.1).

  • Positive control
    • Human kidney and human placenta lysates. Min6 cells transiently expressing mouse CLIC4.



Our Abpromise guarantee covers the use of ab77626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    Peptide ELISA: Antibody detection limit dilution 1/64000.
    ICC/IF: Use at a concentration of 5 µg/ml with overnight incubation.
    WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell-surface expression of HRH3. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis).
    • Tissue specificity
      Detected in epithelial cells from colon, esophagus and kidney (at protein level). Expression is prominent in heart, kidney, placenta and skeletal muscle.
    • Sequence similarities
      Belongs to the chloride channel CLIC family.
      Contains 1 GST C-terminal domain.
    • Domain
      Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.
    • Cellular localization
      Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic vesicle membrane. Nucleus matrix. Cell membrane. Mitochondrion. Cell junction. Colocalized with AKAP9 at the centrosome and midbody. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain. Present in an intracellular vesicular compartment that likely represent trans-Golgi network vesicles.
    • Information by UniProt
    • Database links
    • Alternative names
      • Chloride intracellular channel 4 antibody
      • Chloride intracellular channel 4 (mitochondrial) antibody
      • Chloride intracellular channel 4 like antibody
      • Chloride intracellular channel protein 4 antibody
      • Clic4 antibody
      • CLIC4_HUMAN antibody
      • CLIC4L antibody
      • DKFZP566G223 antibody
      • FLJ38640 antibody
      • H1 antibody
      • HUH1 antibody
      • Intracellular chloride ion channel protein p64H1 antibody
      • MC3S5 antibody
      • mtCLIC antibody
      • p64H1 antibody
      see all


    • Anti-CLIC4 antibody (ab77626) at 0.1 µg/ml + human kidney lysate in RIPA buffer at 35 µg

      Predicted band size: 29 kDa
      Observed band size: 29 kDa

      Primary incubation was 1 hour.
    • Immunofluorescent staining of min6 cells transiently expressing mouse CLIC4 using ab77626 at 5µg/ml with overnight incubation. Nuclear counter staining by Hoechst.


    ab77626 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab76593.

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    DISCOUNT CODE: Expiration date: I am very pleased to hear you would like to accept our offer and test ab77626 inWB on rat. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview...

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