RecombinantRabMAb

Recombinant Anti-CLIC4 antibody [EPR14253] (ab183043)

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Western blot - Anti-CLIC4 antibody [EPR14253] (ab183043)
  • Immunocytochemistry/ Immunofluorescence - Anti-CLIC4 antibody [EPR14253] (ab183043)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLIC4 antibody [EPR14253] (ab183043)
  • Immunoprecipitation - Anti-CLIC4 antibody [EPR14253] (ab183043)
  • Flow Cytometry - Anti-CLIC4 antibody [EPR14253] (ab183043)
  • Immunocytochemistry/ Immunofluorescence - Anti-CLIC4 antibody [EPR14253] (ab183043)
  • Anti-CLIC4 antibody [EPR14253] (ab183043)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14253] to CLIC4
  • Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CLIC4 antibody [EPR14253]
    See all CLIC4 primary antibodies

  • Description

    Rabbit monoclonal [EPR14253] to CLIC4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human CLIC4 aa 1-100. The exact sequence is proprietary.
    Database link: Q9Y696

  • Positive control

    • HeLa, 293, Raji and HepG2 lysates; Human melanoma tissue; HeLa, HepG2 and Raji cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab183043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/500.
IP 1/30 - 1/50.
Flow Cyt 1/90.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell-surface expression of HRH3. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis).

  • Tissue specificity

    Detected in epithelial cells from colon, esophagus and kidney (at protein level). Expression is prominent in heart, kidney, placenta and skeletal muscle.

  • Sequence similarities

    Belongs to the chloride channel CLIC family.
    Contains 1 GST C-terminal domain.

  • Domain

    Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.

  • Cellular localization

    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic vesicle membrane. Nucleus matrix. Cell membrane. Mitochondrion. Cell junction. Colocalized with AKAP9 at the centrosome and midbody. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain. Present in an intracellular vesicular compartment that likely represent trans-Golgi network vesicles.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Chloride intracellular channel 4 antibody
    • Chloride intracellular channel 4 (mitochondrial) antibody
    • Chloride intracellular channel 4 like antibody
    • Chloride intracellular channel protein 4 antibody
    • Clic4 antibody
    • CLIC4_HUMAN antibody
    • CLIC4L antibody
    • DKFZP566G223 antibody
    • FLJ38640 antibody
    • H1 antibody
    • HUH1 antibody
    • Intracellular chloride ion channel protein p64H1 antibody
    • MC3S5 antibody
    • mtCLIC antibody
    • p64H1 antibody
    see all

Images

  • Western blot - Anti-CLIC4 antibody [EPR14253] (ab183043)
    Western blot - Anti-CLIC4 antibody [EPR14253] (ab183043)
    All lanes : Anti-CLIC4 antibody [EPR14253] (ab183043) at 1/10000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : 293 lysate
    Lane 3 : Raji lysate
    Lane 4 : HepG2 lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 29 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CLIC4 antibody [EPR14253] (ab183043)
    Immunocytochemistry/ Immunofluorescence - Anti-CLIC4 antibody [EPR14253] (ab183043)

    Immunofluorescence analysis of HeLa cells (-20°C Acetone-fixed) labeling CLIC4 with ab183043 at 1/500 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLIC4 antibody [EPR14253] (ab183043)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLIC4 antibody [EPR14253] (ab183043)

    Immunohistochemical analysis of paraffin embedded Human melanoma tissue labeling CLIC4 with ab183043 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-CLIC4 antibody [EPR14253] (ab183043)
    Immunoprecipitation - Anti-CLIC4 antibody [EPR14253] (ab183043)

    Western blot analysis of immunoprecipitation pellet from Human Raji cell lysate immunoprecipitated using ab183043 at 1/50 dilution.
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG secondary antibody used at 1/1500 dilution.

  • Flow Cytometry - Anti-CLIC4 antibody [EPR14253] (ab183043)
    Flow Cytometry - Anti-CLIC4 antibody [EPR14253] (ab183043)

    Flow cytometric analysis of Raji cells (paraformaldehyde-fixed, 2%) labeling CLIC4 with ab183043 at 1/90 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-CLIC4 antibody [EPR14253] (ab183043)
    Immunocytochemistry/ Immunofluorescence - Anti-CLIC4 antibody [EPR14253] (ab183043)

    Immunofluorescence analysis of HepG2 cells (paraformaldehyde-fixed, 4%) labeling CLIC4 with ab183043 at 1/500 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Anti-CLIC4 antibody [EPR14253] (ab183043)
    Anti-CLIC4 antibody [EPR14253] (ab183043)

References (1)

Publishing research using ab183043? Please let us know so that we can cite the reference in this datasheet.

ab183043 has been referenced in 1 publication.

  • Feng Z  et al. Chloride intracellular channel 4 participate in the protective effect of Ginkgolide B in MPP+ injured MN9D cells: insight from proteomic analysis. Clin Proteomics 17:32 (2020). PubMed: 32944011

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