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    clic4-antibody-epr14253-ab183043.pdf

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RecombinantRabMAb

Recombinant Anti-CLIC4 antibody [EPR14253] (ab183043)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Western blot - Anti-CLIC4 antibody [EPR14253] (ab183043)
  • Immunocytochemistry/ Immunofluorescence - Anti-CLIC4 antibody [EPR14253] (ab183043)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLIC4 antibody [EPR14253] (ab183043)
  • Immunoprecipitation - Anti-CLIC4 antibody [EPR14253] (ab183043)
  • Flow Cytometry (Intracellular) - Anti-CLIC4 antibody [EPR14253] (ab183043)
  • Immunocytochemistry/ Immunofluorescence - Anti-CLIC4 antibody [EPR14253] (ab183043)
  • Anti-CLIC4 antibody [EPR14253] (ab183043)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14253] to CLIC4
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

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Overview

  • Product name

    Anti-CLIC4 antibody [EPR14253]
    See all CLIC4 primary antibodies
  • Description

    Rabbit monoclonal [EPR14253] to CLIC4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, 293, Raji and HepG2 lysates; Human melanoma tissue; HeLa, HepG2 and Raji cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14253
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Nucleus
    • Other
    • Cell Biology
    • Apoptosis
    • Mitochondrial
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Apoptosis
    • Nuclear
    • Signal Transduction
    • Metabolism
    • Vitamins / Minerals
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Vitamins / minerals
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Mitochondrial
    • Cancer
    • Cell Death
    • Apoptosis
    • Nucleus
    • Other
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Alternative Versions

    • PE Anti-CLIC4 antibody [EPR14253] (ab212152)
    • Anti-CLIC4 antibody [EPR14253] - BSA and Azide free (ab236130)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Raji whole cell lysate (ab30124)
  • Recombinant Protein

    • Recombinant Human CLIC4 protein (ab104744)
  • Related Products

    • Recombinant Human CLIC4 protein (ab104744)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab183043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/90.

ab172730- Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/10000 - 1/50000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/500.
IP
1/30 - 1/50.
Notes
Flow Cyt (Intra)
1/90.

ab172730- Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/10000 - 1/50000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/500.
IP
1/30 - 1/50.

Target

  • Function

    Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell-surface expression of HRH3. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis).
  • Tissue specificity

    Detected in epithelial cells from colon, esophagus and kidney (at protein level). Expression is prominent in heart, kidney, placenta and skeletal muscle.
  • Sequence similarities

    Belongs to the chloride channel CLIC family.
    Contains 1 GST C-terminal domain.
  • Domain

    Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.
  • Cellular localization

    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic vesicle membrane. Nucleus matrix. Cell membrane. Mitochondrion. Cell junction. Colocalized with AKAP9 at the centrosome and midbody. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain. Present in an intracellular vesicular compartment that likely represent trans-Golgi network vesicles.
  • Target information above from: UniProt accession Q9Y696 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 25932 Human
    • Entrez Gene: 29876 Mouse
    • Entrez Gene: 83718 Rat
    • Omim: 606536 Human
    • SwissProt: Q9Y696 Human
    • SwissProt: Q9QYB1 Mouse
    • SwissProt: Q9Z0W7 Rat
    • Unigene: 440544 Human
    • Unigene: 257765 Mouse
    • Unigene: 104756 Rat
    see all
  • Alternative names

    • Chloride intracellular channel 4 antibody
    • Chloride intracellular channel 4 (mitochondrial) antibody
    • Chloride intracellular channel 4 like antibody
    • Chloride intracellular channel protein 4 antibody
    • Clic4 antibody
    • CLIC4_HUMAN antibody
    • CLIC4L antibody
    • DKFZP566G223 antibody
    • FLJ38640 antibody
    • H1 antibody
    • HUH1 antibody
    • Intracellular chloride ion channel protein p64H1 antibody
    • MC3S5 antibody
    • mtCLIC antibody
    • p64H1 antibody
    see all

Images

  • Western blot - Anti-CLIC4 antibody [EPR14253] (ab183043)
    Western blot - Anti-CLIC4 antibody [EPR14253] (ab183043)
    All lanes : Anti-CLIC4 antibody [EPR14253] (ab183043) at 1/10000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : 293 lysate
    Lane 3 : Raji lysate
    Lane 4 : HepG2 lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 29 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CLIC4 antibody [EPR14253] (ab183043)
    Immunocytochemistry/ Immunofluorescence - Anti-CLIC4 antibody [EPR14253] (ab183043)

    Immunofluorescence analysis of HeLa cells (-20°C Acetone-fixed) labeling CLIC4 with ab183043 at 1/500 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLIC4 antibody [EPR14253] (ab183043)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLIC4 antibody [EPR14253] (ab183043)

    Immunohistochemical analysis of paraffin embedded Human melanoma tissue labeling CLIC4 with ab183043 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-CLIC4 antibody [EPR14253] (ab183043)
    Immunoprecipitation - Anti-CLIC4 antibody [EPR14253] (ab183043)

    Western blot analysis of immunoprecipitation pellet from Human Raji cell lysate immunoprecipitated using ab183043 at 1/50 dilution.
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG secondary antibody used at 1/1500 dilution.

  • Flow Cytometry (Intracellular) - Anti-CLIC4 antibody [EPR14253] (ab183043)
    Flow Cytometry (Intracellular) - Anti-CLIC4 antibody [EPR14253] (ab183043)

    Intracellular flow cytometric analysis ofRaji cells (paraformaldehyde-fixed, 2%)labeling CLIC4 with ab183043 at 1/90 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution. 

     

     

  • Immunocytochemistry/ Immunofluorescence - Anti-CLIC4 antibody [EPR14253] (ab183043)
    Immunocytochemistry/ Immunofluorescence - Anti-CLIC4 antibody [EPR14253] (ab183043)

    Immunofluorescence analysis of HepG2 cells (paraformaldehyde-fixed, 4%) labeling CLIC4 with ab183043 at 1/500 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Anti-CLIC4 antibody [EPR14253] (ab183043)
    Anti-CLIC4 antibody [EPR14253] (ab183043)

Protocols

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (3)

Publishing research using ab183043? Please let us know so that we can cite the reference in this datasheet.

ab183043 has been referenced in 3 publications.

  • Shao X  et al. Astragaloside IV alleviates atherosclerosis through targeting circ_0000231/miR-135a-5p/CLIC4 axis in AS cell model in vitro. Mol Cell Biochem 476:1783-1795 (2021). PubMed: 33439448
  • Feng Z  et al. Chloride intracellular channel 4 participate in the protective effect of Ginkgolide B in MPP+ injured MN9D cells: insight from proteomic analysis. Clin Proteomics 17:32 (2020). PubMed: 32944011
  • Ling CK  et al. Chloride intracellular channel 4 is dysregulated in endometrium of women with infertility and alters receptivity. Biochem Biophys Res Commun 531:490-496 (2020). PubMed: 32807494

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