Recombinant
RabMAb

Recombinant Anti-CLIC4 antibody [EPR14253] - BSA and Azide free (ab236130)

Overview

  • Product name

    Anti-CLIC4 antibody [EPR14253] - BSA and Azide free
    See all CLIC4 primary antibodies
  • Description

    Rabbit monoclonal [EPR14253] to CLIC4 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IP, ICC/IF, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CLIC4 aa 1-100. The exact sequence is proprietary.
    Database link: Q9Y696

  • Positive control

    • IHC-P: Human melanoma tissue.
  • General notes

    Ab236130 is the carrier-free version of ab183043. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab236130 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab236130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB Use at an assay dependent concentration. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).

Target

  • Function

    Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell-surface expression of HRH3. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis).
  • Tissue specificity

    Detected in epithelial cells from colon, esophagus and kidney (at protein level). Expression is prominent in heart, kidney, placenta and skeletal muscle.
  • Sequence similarities

    Belongs to the chloride channel CLIC family.
    Contains 1 GST C-terminal domain.
  • Domain

    Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.
  • Cellular localization

    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic vesicle membrane. Nucleus matrix. Cell membrane. Mitochondrion. Cell junction. Colocalized with AKAP9 at the centrosome and midbody. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain. Present in an intracellular vesicular compartment that likely represent trans-Golgi network vesicles.
  • Information by UniProt
  • Database links

  • Alternative names

    • Chloride intracellular channel 4 antibody
    • Chloride intracellular channel 4 (mitochondrial) antibody
    • Chloride intracellular channel 4 like antibody
    • Chloride intracellular channel protein 4 antibody
    • Clic4 antibody
    • CLIC4_HUMAN antibody
    • CLIC4L antibody
    • DKFZP566G223 antibody
    • FLJ38640 antibody
    • H1 antibody
    • HUH1 antibody
    • Intracellular chloride ion channel protein p64H1 antibody
    • MC3S5 antibody
    • mtCLIC antibody
    • p64H1 antibody
    see all

Images

  • Western blot analysis of immunoprecipitation pellet from Human Raji cell lysate immunoprecipitated using ab183043 at 1/50 dilution.
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG secondary antibody used at 1/1500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183043).

  • Flow cytometric analysis of Raji cells (paraformaldehyde-fixed, 2%) labeling CLIC4 with ab183043 at 1/90 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183043).

  • Immunofluorescence analysis of HepG2 cells (paraformaldehyde-fixed, 4%) labeling CLIC4 with ab183043 at 1/500 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183043).

  • Immunofluorescence analysis of HeLa cells (-20°C Acetone-fixed) labeling CLIC4 with ab183043 at 1/500 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183043).

  • Immunohistochemical analysis of paraffin embedded Human melanoma tissue labeling CLIC4 with ab183043 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183043).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

References

ab236130 has not yet been referenced specifically in any publications.

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