RecombinantRabMAb

Recombinant Anti-CLIC4 antibody [EPR14253] - BSA and Azide free (ab236130)

Submit a review Submit a question
Immunoprecipitation - Anti-CLIC4 antibody [EPR14253] - BSA and Azide free (ab236130)
  • Flow Cytometry - Anti-CLIC4 antibody [EPR14253] - BSA and Azide free (ab236130)
  • Immunocytochemistry/ Immunofluorescence - Anti-CLIC4 antibody [EPR14253] - BSA and Azide free (ab236130)
  • Immunocytochemistry/ Immunofluorescence - Anti-CLIC4 antibody [EPR14253] - BSA and Azide free (ab236130)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLIC4 antibody [EPR14253] - BSA and Azide free (ab236130)
  • Anti-CLIC4 antibody [EPR14253] - BSA and Azide free (ab236130)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14253] to CLIC4 - BSA and Azide free
  • Suitable for: IHC-P, IP, ICC/IF, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CLIC4 antibody [EPR14253] - BSA and Azide free
    See all CLIC4 primary antibodies

  • Description

    Rabbit monoclonal [EPR14253] to CLIC4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IP, ICC/IF, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human CLIC4 aa 1-100. The exact sequence is proprietary.
    Database link: Q9Y696

  • Positive control

    • IHC-P: Human melanoma tissue.

  • General notes

    Ab236130 is the carrier-free version of ab183043. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab236130 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Applications

Our Abpromise guarantee covers the use of ab236130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB Use at an assay dependent concentration. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).

Target

  • Function

    Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell-surface expression of HRH3. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis).

  • Tissue specificity

    Detected in epithelial cells from colon, esophagus and kidney (at protein level). Expression is prominent in heart, kidney, placenta and skeletal muscle.

  • Sequence similarities

    Belongs to the chloride channel CLIC family.
    Contains 1 GST C-terminal domain.

  • Domain

    Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.

  • Cellular localization

    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic vesicle membrane. Nucleus matrix. Cell membrane. Mitochondrion. Cell junction. Colocalized with AKAP9 at the centrosome and midbody. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain. Present in an intracellular vesicular compartment that likely represent trans-Golgi network vesicles.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Chloride intracellular channel 4 antibody
    • Chloride intracellular channel 4 (mitochondrial) antibody
    • Chloride intracellular channel 4 like antibody
    • Chloride intracellular channel protein 4 antibody
    • Clic4 antibody
    • CLIC4_HUMAN antibody
    • CLIC4L antibody
    • DKFZP566G223 antibody
    • FLJ38640 antibody
    • H1 antibody
    • HUH1 antibody
    • Intracellular chloride ion channel protein p64H1 antibody
    • MC3S5 antibody
    • mtCLIC antibody
    • p64H1 antibody
    see all

Images

  • Immunoprecipitation - Anti-CLIC4 antibody [EPR14253] - BSA and Azide free (ab236130)
    Immunoprecipitation - Anti-CLIC4 antibody [EPR14253] - BSA and Azide free (ab236130)

    Western blot analysis of immunoprecipitation pellet from Human Raji cell lysate immunoprecipitated using ab183043 at 1/50 dilution.
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG secondary antibody used at 1/1500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183043).

  • Flow Cytometry - Anti-CLIC4 antibody [EPR14253] - BSA and Azide free (ab236130)
    Flow Cytometry - Anti-CLIC4 antibody [EPR14253] - BSA and Azide free (ab236130)

    Flow cytometric analysis of Raji cells (paraformaldehyde-fixed, 2%) labeling CLIC4 with ab183043 at 1/90 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183043).

  • Immunocytochemistry/ Immunofluorescence - Anti-CLIC4 antibody [EPR14253] - BSA and Azide free (ab236130)
    Immunocytochemistry/ Immunofluorescence - Anti-CLIC4 antibody [EPR14253] - BSA and Azide free (ab236130)

    Immunofluorescence analysis of HepG2 cells (paraformaldehyde-fixed, 4%) labeling CLIC4 with ab183043 at 1/500 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183043).

  • Immunocytochemistry/ Immunofluorescence - Anti-CLIC4 antibody [EPR14253] - BSA and Azide free (ab236130)
    Immunocytochemistry/ Immunofluorescence - Anti-CLIC4 antibody [EPR14253] - BSA and Azide free (ab236130)

    Immunofluorescence analysis of HeLa cells (-20°C Acetone-fixed) labeling CLIC4 with ab183043 at 1/500 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183043).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLIC4 antibody [EPR14253] - BSA and Azide free (ab236130)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLIC4 antibody [EPR14253] - BSA and Azide free (ab236130)

    Immunohistochemical analysis of paraffin embedded Human melanoma tissue labeling CLIC4 with ab183043 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183043).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-CLIC4 antibody [EPR14253] - BSA and Azide free (ab236130)
    Anti-CLIC4 antibody [EPR14253] - BSA and Azide free (ab236130)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab236130? Please let us know so that we can cite the reference in this datasheet.

ab236130 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab236130.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com