Overview

  • Product name

    Anti-CLIC5 antibody [CLIC502]
    See all CLIC5 primary antibodies
  • Description

    Mouse monoclonal [CLIC502] to CLIC5
  • Host species

    Mouse
  • Specificity

    ab191102 recognizes CLIC5a, a 32 kDa protein which is component of actin complexes. Cross reactivity with CLIC5b was not determined.
  • Tested applications

    Suitable for: Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human CLIC5 aa 160-173 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    IDANTCGEDKGSRR


    Database link: Q9NZA1-3

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.097% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    ab191102 was purified from cell culture supernatant by protein-A affinity chromatography.
  • Clonality

    Monoclonal
  • Clone number

    CLIC502
  • Isotype

    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa.

Target

  • Function

    Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. May play a role in the regulation of transepithelial ion absorption and secretion. Required for normal formation of stereocilia in the inner ear and normal development of the organ of Corti (By similarity). Is required for the development and/or maintenance of the proper glomerular endothelial cell and podocyte architecture.
  • Tissue specificity

    Isoform 1: Expressed in renal glomeruli endothelial cells and podocytes (at protein level).
  • Sequence similarities

    Belongs to the chloride channel CLIC family.
    Contains 1 GST C-terminal domain.
  • Domain

    Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.
  • Cellular localization

    Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Membrane. Associates with the cortical actin cytoskeleton. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain and Golgi apparatus. Cytoplasm > cytoskeleton > centrosome. Membrane. Colocalized with AKAP9 at the Golgi apparatus as well as, to a lesser extent, the centrosome. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain (By similarity). Colocalized with podocalyxin in renal glomeruli.
  • Information by UniProt
  • Database links

  • Alternative names

    • Chloride intracellular channel 5 antibody
    • Chloride intracellular channel 5A antibody
    • Chloride intracellular channel protein 5 antibody
    • CLIC 5 antibody
    • CLIC 5B antibody
    • CLIC5 antibody
    • CLIC5_HUMAN antibody
    • CLIC5B antibody
    • dJ447E21.4 antibody
    • FLJ90663 antibody
    • MST 130 antibody
    • MST130 antibody
    • MSTP 130 antibody
    • MSTP130 antibody
    see all

Images

  • All lanes : Anti-CLIC5 antibody [CLIC502] (ab191102)

    Lane 1 : HEK293 cell lysate, transfected with CLIC5
    Lane 2 : HEK293 cell lysate

    Predicted band size: 28 kDa

References

This product has been referenced in:

See 1 Publication for this product

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