Key features and details
- Rabbit polyclonal to CLINT1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-CLINT1 antibody
See all CLINT1 primary antibodies
DescriptionRabbit polyclonal to CLINT1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Gorilla, Orangutan, Platypus
Synthetic peptide corresponding to a region between residues 250 and 300 of Human CLINT1 (NP_055481.1).
- HeLa, 293T and NIH3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab86046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 68 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionBinds to membranes enriched in phosphatidylinositol-4,5-biphosphate (PtdIns(4,5)P2). May have a role in transport via clathrin-coated vesicles from the trans-Golgi network to endosomes. Stimulates clathrin assembly.
Tissue specificityUbiquitously expressed at low to intermediate levels.
Sequence similaritiesBelongs to the epsin family.
Contains 1 ENTH (epsin N-terminal homology) domain.
Cellular localizationCytoplasm. Cytoplasm > perinuclear region. Membrane. Cytoplasmic vesicle > clathrin-coated vesicle. Found throughout the cell, with the exception of the cell surface. Concentrated in the perinuclear region and associated with clathrin-coated vesicles close to the trans-Golgi network.
- Information by UniProt
- Clathrin interacting protein antibody
- Clathrin interactor 1 antibody
- Clathrin-interacting protein localized in the trans-Golgi region antibody
All lanes : Anti-CLINT1 antibody (ab86046) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 68 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
Exposure time: 3 seconds
Detection of Human CLINT1 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86046 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 10 seconds.
ab86046 has not yet been referenced specifically in any publications.