Key features and details
- Mouse monoclonal [mAbcam61830] to CLIP170
- Suitable for: Flow Cyt, ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-CLIP170 antibody [mAbcam61830]
See all CLIP170 primary antibodies
DescriptionMouse monoclonal [mAbcam61830] to CLIP170
Tested applicationsSuitable for: Flow Cyt, ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment corresponding to Human CLIP170 aa 1100 to the C-terminus.
(Peptide available as
- This antibody gave a positive signal in human skeletal muscle tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Concentration information loading...
Our Abpromise guarantee covers the use of ab61830 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use a concentration of 10 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 161 kDa (predicted molecular weight: 161 kDa).|
|IHC-P||Use a concentration of 5 mg/ml.|
FunctionSeems to be a intermediate filament associated protein that links endocytic vesicles to microtubules.
Tissue specificityHighly expressed in the Reed-Sternberg cells of Hodgkin disease.
Sequence similaritiesContains 2 CAP-Gly domains.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. Associated with the cytoskeleton. Localizes to the tips of microtubules.
- Information by UniProt
- CAP GLY domain containing linker protein 1 antibody
- CAP-Gly domain-containing linker protein 1 antibody
- CLIP 170 antibody
Anti-CLIP170 antibody [mAbcam61830] (ab61830) at 1 µg/ml + Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 161 kDa
Observed band size: 161 kDa
Additional bands at: 190 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
ICC/IF image of ab61830 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab61830 at 10µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in HeLa, HepG2, and MCF-7 cell lines in PFA at 10ug/ml
IHC image of Ab61830 staining in Human Cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Ab61830, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Overlay histogram showing HeLa cells stained with ab61830 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab61830, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
ab61830 has not yet been referenced specifically in any publications.