Product nameAnti-CLIP4 antibody
DescriptionRabbit polyclonal to CLIP4
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
- IHC-P: Human smooth muscle tissue. WB: Human tonsil lysate. ICC/IF: U-2 OS cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab243532 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.4 µg/ml. Predicted molecular weight: 76 kDa.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100
Sequence similaritiesContains 3 ANK repeats.
Contains 3 CAP-Gly domains.
- Information by UniProt
- CAP GLY domain containing linker protein family member 4 antibody
- CAP-Gly domain-containing linker protein 4 antibody
- Clip4 antibody
PFA fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells labeling CLIP4 using ab243532 at 4 µg/ml (green) in ICC/IF. Nuclear probe is visualized in blue, microtublues in red.
All lanes : Anti-CLIP4 antibody (ab243532) at 0.4 µg/ml
Lane 1 : RT4 (Human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (Human brain glioma cell line) cell lysate
Lane 3 : Human plasma (IgG/HSA depleted)
Lane 4 : Human liver tissue
Lane 5 : Human tonsil tissue
Predicted band size: 76 kDa
Formalin-fixed, paraffin-embedded human smooth muscle tissue stained for CLIP4 with ab243532 at 1/20 dilution in immunohistochemical analysis.
ab243532 has not yet been referenced specifically in any publications.