Key features and details
- Rabbit polyclonal to CLIP4
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CLIP4 antibody
DescriptionRabbit polyclonal to CLIP4
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
- IHC-P: Human smooth muscle tissue. WB: Human tonsil lysate. ICC/IF: U-2 OS cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab243532 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 76 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
Sequence similaritiesContains 3 ANK repeats.
Contains 3 CAP-Gly domains.
- Information by UniProt
- CAP GLY domain containing linker protein family member 4 antibody
- CAP-Gly domain-containing linker protein 4 antibody
- Clip4 antibody
PFA fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells labeling CLIP4 using ab243532 at 4 µg/ml (green) in ICC/IF. Nuclear probe is visualized in blue, microtublues in red.
All lanes : Anti-CLIP4 antibody (ab243532) at 0.4 µg/ml
Lane 1 : RT4 (Human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (Human brain glioma cell line) cell lysate
Lane 3 : Human plasma (IgG/HSA depleted)
Lane 4 : Human liver tissue
Lane 5 : Human tonsil tissue
Predicted band size: 76 kDa
Formalin-fixed, paraffin-embedded human smooth muscle tissue stained for CLIP4 with ab243532 at 1/20 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab243532 has not yet been referenced specifically in any publications.