Key features and details
- Rabbit polyclonal to CLK2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CLK2 antibody
See all CLK2 primary antibodies
DescriptionRabbit polyclonal to CLK2
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
- IHC-P: Human colon, testis, and skin tissues; WB: BeWo cell lysate; ICC/IF: U-2 OS cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab188141 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 60 kDa.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionPhosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates serines, threonines and tyrosines.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.
Contains 1 protein kinase domain.
modificationsAutophosphorylates on all three types of residues.
- Information by UniProt
- CDC like kinase 2 antibody
- CDC-like kinase 2 antibody
- CLK 2 antibody
Anti-CLK2 antibody (ab188141) at 0.4 µg/ml + BeWo (human placenta choriocarcinoma cell line) cell lysate
Predicted band size: 60 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling CLK2 with ab188141 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemical analysis of PFA/Triton X-100 fixed U-2 OS cells labeling CLK2 with ab188141 at 2 µg/ml.
Paraffin embedded human colon tissue stained for CLK2 using ab188141 at 1/200 dilution in immunohistochemical analysis.
Paraffin embedded human skin tissue stained for CLK2 using ab188141 at 1/200 dilution in immunohistochemical analysis.
Paraffin embedded human skeletal muscle tissue stained for CLK2 using ab188141 at 1/200 dilution in immunohistochemical analysis. Shows no positivity in myocytes cells as expected.
ab188141 has been referenced in 2 publications.
- Kulkarni P et al. Phosphorylation-induced conformational dynamics in an intrinsically disordered protein and potential role in phenotypic heterogeneity. Proc Natl Acad Sci U S A 114:E2644-E2653 (2017). PubMed: 28289210
- Park SY et al. Cdc2-like kinase 2 is a key regulator of the cell cycle via FOXO3a/p27 in glioblastoma. Oncotarget 7:26793-805 (2016). PubMed: 27050366