Overview

  • Product name

  • Description

    Rabbit polyclonal to CLK2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment corresponding to Human CLK2 aa 3-66.
    Sequence:

    HPRRYHSSERGSRGSYREHYRSRKHKRRRSRSWSSSSDRTRRRRREDSYH VRSRSSYDDRSSDR


    Database link: P49760

  • Positive control

    • Human colon tissue, RT-4 and U-251MG lysates, U-2OS cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab188141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.
WB 1/100 - 1/250. Predicted molecular weight: 60 kDa.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates serines, threonines and tyrosines.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylates on all three types of residues.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CDC like kinase 2 antibody
    • CDC-like kinase 2 antibody
    • CLK 2 antibody
    • CLK kinase antibody
    • CLK2 antibody
    • Clk2 Scamp3 antibody
    • CLK2_HUMAN antibody
    • Dual specificity protein kinase CLK2 antibody
    • hCLK 2 antibody
    • hCLK2 antibody
    • Tu52 antibody
    see all

Images

  • All lanes : Anti-CLK2 antibody (ab188141) at 1/100 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251MG cell lysate

    Predicted band size: 60 kDa

  • Immunohistochemical analysis of human colon tissue labelling CLK2 with ab188141. Moderate to strong nuclear positivty is shown.

  • Immunocytochemical analysis of PFA/Triton X-100 fixed U-2OS cells labeling CLK2 with ab188141 at 4µg/ml.

  • Immunohistochemical analysis of human kindye tissue labelling CLK2 with ab188141. Moderate to strong nuclear positivty is shown.

  • Immunohistochemical analysis of skin tissue labelling CLK2 with ab188141. Moderate nuclear positivty in squamous epithelia is shown.

  • Immunohistochemical analysis of formalin fixed paraffin embedded human colon tissue labeling CLK2 with ab188141 at 1/50 dilution

References

This product has been referenced in:

  • Kulkarni P  et al. Phosphorylation-induced conformational dynamics in an intrinsically disordered protein and potential role in phenotypic heterogeneity. Proc Natl Acad Sci U S A 114:E2644-E2653 (2017). Read more (PubMed: 28289210) »
  • Park SY  et al. Cdc2-like kinase 2 is a key regulator of the cell cycle via FOXO3a/p27 in glioblastoma. Oncotarget 7:26793-805 (2016). Read more (PubMed: 27050366) »
See all 2 Publications for this product

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