Overview

  • Product name

  • Description

    Rabbit polyclonal to CLK2
  • Host species

    Rabbit
  • Specificity

    ab65082 detects endogenous levels of total CLK2 protein.
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide derived from an internal region of human CLK2.

  • Positive control

    • Extracts from COLO205 cells and A549 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab65082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
ELISA 1/20000.

Target

  • Function

    Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates serines, threonines and tyrosines.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylates on all three types of residues.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CDC like kinase 2 antibody
    • CDC-like kinase 2 antibody
    • CLK 2 antibody
    • CLK kinase antibody
    • CLK2 antibody
    • Clk2 Scamp3 antibody
    • CLK2_HUMAN antibody
    • Dual specificity protein kinase CLK2 antibody
    • hCLK 2 antibody
    • hCLK2 antibody
    • Tu52 antibody
    see all

Images

  • All lanes : Anti-CLK2 antibody (ab65082) at 1/500 dilution

    Lane 1 : Extracts from COLO205 cells
    Lane 2 : Extracts from A549 cells
    Lane 3 : Extracts from A549 cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 60 kDa
    Observed band size: 60 kDa

  • Anti-CLK2 antibody (ab65082) at 1/1000 dilution (in milk for 18 hours at 4°C) + HeLa cell nuclear lysate at 50 µg

    Secondary
    An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 60 kDa
    Observed band size: 60 kDa


    Exposure time: 1 minute


    Blocking Step: 5% Milk for 1 hour at 22°C

    See Abreview

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (Human mammary cell)
Loading amount
50000 cells
Specification
Human mammary cell
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Apr 17 2012

Answer

I am very pleased to hear you would like to accept our offer and test ab65082 and/or ab86147 in immunoprecipitation. This code will give you one free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for immunoprecipitation and include the appropriate code in the “Additional Comments” section so we know the Abreview is for this promotion.

For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

We publish positive and negative Abreviews on our datasheets so please submit the results of your tests regardless of the outcome. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for your patience. None of our CLK antibodies have been tested against purified protein of other CLK family members. Cross-reactivity of, for instance, a CLK1 antibody with CLK2, 3, or 4 has not been confirmed. The best we can do is predict the potential for cross-reactivity by aligning the immunogens of each antibody, when they are available, with the sequences of the other proteins. The N terminus appears to have a lot of diversity among these four, so antibodies raised against the N terminus are likely to have specificity for their targets. For example, the CLK2 antibody ab86147 is raised against the CLK2 N terminus which has little or no homology with the N termini of CLK1, 3, or 4. This is also the case for the CLK3 antibody ab37919, raised against the N terminus of CLK3. We do not have the immunogen sequence information for the CLK1 and CLK4 antibodies in our catalogue, but I can obtain alignment scores for these if you are still interested. For proteins, we only have a CLK1 protein, ab114706, full-length recombinant human with a 26 kDa GST tag. There is also a CLK2 protein fragment, but it does not include the N terminus. I hope this helps, at least a little. Please let me know if you need more advice or information.

Read More

Answer

Thank you for contacting us. I am very sorry for the confusion. I performed a BLAST to find out the percentage of homology between the four proteins, and the results are: -CLK1-CLK2: 53.5% -CLK1-CLK3: 14% -CLK1-CLK4: 77% -CLK2-CLK3: 17% -CLK2-CLK4: 55% -CLK3-CLK4: 14% We usually predict cross-reactivity when the homology between the sequences is over 85%. So, in this case, I would dare to affirm there might be some slight reactivity between CLK1 and CLK4, but the rest of proteins are very unlikely to cross react. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More
Application
Western blot
Sample
Human Cell lysate - whole cell (Colon cell line)
Loading amount
20 µg
Specification
Colon cell line
Gel Running Conditions
Reduced Denaturing (3-8%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 03 2011

Application
Western blot
Sample
Human Cell lysate - nuclear (HeLa)
Loading amount
50 µg
Specification
HeLa
Gel Running Conditions
Reduced Denaturing (10% Tris-Bicine)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Aug 10 2010

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