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    clk2-antibody-ab86147.pdf

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Cell Biology Cell Cycle Kinases/Phosphatases Other
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Anti-CLK2 antibody (ab86147)

  • Datasheet
Reviews (4)Q&A (1)References (2)

Product price, shipping and contact information

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Immunocytochemistry/ Immunofluorescence - Anti-CLK2 antibody (ab86147)
  • Western blot - Anti-CLK2 antibody (ab86147)
  • Immunocytochemistry/ Immunofluorescence - Anti-CLK2 antibody (ab86147)

Key features and details

  • Rabbit polyclonal to CLK2
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-CLK2 antibody
    See all CLK2 primary antibodies
  • Description

    Rabbit polyclonal to CLK2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chinese hamster
  • Immunogen

    Synthetic peptide corresponding to Human CLK2 aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab99082)

  • Positive control

    • This antibody gave a positive signal in HeLa whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Other
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Splicing

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Human CLK2 peptide (ab99082)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human CLK2 protein (ab63190)

Applications

Our Abpromise guarantee covers the use of ab86147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates serines, threonines and tyrosines.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylates on all three types of residues.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P49760 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1196 Human
    • Entrez Gene: 12748 Mouse
    • Entrez Gene: 365842 Rat
    • Omim: 602989 Human
    • SwissProt: P49760 Human
    • SwissProt: O35491 Mouse
    • Unigene: 73986 Human
    • Unigene: 288098 Mouse
    • Unigene: 17201 Rat
    see all
  • Alternative names

    • CDC like kinase 2 antibody
    • CDC-like kinase 2 antibody
    • CLK 2 antibody
    • CLK kinase antibody
    • CLK2 antibody
    • Clk2 Scamp3 antibody
    • CLK2_HUMAN antibody
    • Dual specificity protein kinase CLK2 antibody
    • hCLK 2 antibody
    • hCLK2 antibody
    • Tu52 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-CLK2 antibody (ab86147)
    Immunocytochemistry/ Immunofluorescence - Anti-CLK2 antibody (ab86147)This image is courtesy of an Abreview submitted by Eleni Petsalaki

    ab86147 staining CLK2 in human colon carcinoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and blocked with 1% BSA for 30 minutes at 37°C. Samples were incubated with primary antibody (1/30 in PBS + 1% BSA) for 16 hour at 4°C. A FITC-conjugated goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.

    See Abreview

  • Western blot - Anti-CLK2 antibody (ab86147)
    Western blot - Anti-CLK2 antibody (ab86147)
    Anti-CLK2 antibody (ab86147) at 0.5 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 60 kDa
    Observed band size: 60 kDa
    Additional bands at: 36 kDa, 64 kDa, 94 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes


    Human Dual specificity protein kinase CLK2 contains a number of potential phosphorylation sites (SwissProt) which may explain the banding pattern at a higher molecular weight than predicted (64 kDa).
  • Immunocytochemistry/ Immunofluorescence - Anti-CLK2 antibody (ab86147)
    Immunocytochemistry/ Immunofluorescence - Anti-CLK2 antibody (ab86147)

    ICC/IF image of ab86147 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab86147 at 5ug overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti- Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in HeLa pfa fixed cell types at 5ug/ml

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (2)

    Publishing research using ab86147? Please let us know so that we can cite the reference in this datasheet.

    ab86147 has been referenced in 2 publications.

    • Iwai K  et al. Anti-tumor efficacy of a novel CLK inhibitor via targeting RNA splicing and MYC-dependent vulnerability. EMBO Mol Med 10:N/A (2018). PubMed: 29769258
    • Petsalaki E & Zachos G Clks 1, 2 and 4 prevent chromatin breakage by regulating the Aurora B-dependent abscission checkpoint. Nat Commun 7:11451 (2016). PubMed: 27126587

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-5 of 5 Abreviews or Q&A

    Western blot abreview for Anti-CLK2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Non-reduced Denaturing (10)
    Sample
    Human Cell lysate - whole cell (Colon carcinoma cells)
    Specification
    Colon carcinoma cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. Eleni Petsalaki

    Verified customer

    Submitted Apr 18 2014

    Western blot abreview for Anti-CLK2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Non-reduced Denaturing (10)
    Sample
    Hamster Cell lysate - whole cell (Chinese Hamster Ovary cells)
    Specification
    Chinese Hamster Ovary cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 25°C
    Read More

    Dr. Eleni Petsalaki

    Verified customer

    Submitted Apr 02 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CLK2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C
    Sample
    Hamster Cell (Chinese Hamster Ovary cells)
    Specification
    Chinese Hamster Ovary cells
    Permeabilization
    Yes - 0.5% Triton
    Fixative
    Methanol
    Read More

    Dr. Eleni Petsalaki

    Verified customer

    Submitted Apr 01 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CLK2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C
    Sample
    Human Cell (Colon carcinoma cells)
    Specification
    Colon carcinoma cells
    Permeabilization
    No
    Fixative
    Methanol
    Read More

    Dr. Eleni Petsalaki

    Verified customer

    Submitted Mar 24 2014

    Question

    Please send testing discount codes for immunoprecipitation of CLK2 with ab65082 and ab86147.

    Read More

    Abcam community

    Verified customer

    Asked on Apr 02 2012

    Answer

    I am very pleased to hear you would like to accept our offer and test ab65082 and/or ab86147 in immunoprecipitation. This code will give you one free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for immunoprecipitation and include the appropriate code in the “Additional Comments” section so we know the Abreview is for this promotion.

    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    We publish positive and negative Abreviews on our datasheets so please submit the results of your tests regardless of the outcome. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Apr 02 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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