Anti-CLK2 antibody (ab86147)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-CLK2 antibody
See all CLK2 primary antibodies -
Description
Rabbit polyclonal to CLK2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human, Chinese hamster
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to Human CLK2 aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab99082) -
Positive control
- This antibody gave a positive signal in HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab86147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). | |
| ICC/IF | Use a concentration of 5 µg/ml. |
Target
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Function
Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates serines, threonines and tyrosines. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylates on all three types of residues. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1196 Human
- Entrez Gene: 12748 Mouse
- Entrez Gene: 365842 Rat
- Omim: 602989 Human
- SwissProt: P49760 Human
- SwissProt: O35491 Mouse
- Unigene: 73986 Human
- Unigene: 288098 Mouse
see all -
Alternative names
- CDC like kinase 2 antibody
- CDC-like kinase 2 antibody
- CLK 2 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CLK2 antibody (ab86147)This image is courtesy of an Abreview submitted by Eleni Petsalakiab86147 staining CLK2 in human colon carcinoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and blocked with 1% BSA for 30 minutes at 37°C. Samples were incubated with primary antibody (1/30 in PBS + 1% BSA) for 16 hour at 4°C. A FITC-conjugated goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.
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Western blot - Anti-CLK2 antibody (ab86147)Anti-CLK2 antibody (ab86147) at 0.5 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 60 kDa
Additional bands at: 36 kDa, 64 kDa, 94 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
Human Dual specificity protein kinase CLK2 contains a number of potential phosphorylation sites (SwissProt) which may explain the banding pattern at a higher molecular weight than predicted (64 kDa). -
Immunocytochemistry/ Immunofluorescence - Anti-CLK2 antibody (ab86147)ICC/IF image of ab86147 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab86147 at 5ug overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti- Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in HeLa pfa fixed cell types at 5ug/ml
Datasheets and documents
References
This product has been referenced in:
- Iwai K et al. Anti-tumor efficacy of a novel CLK inhibitor via targeting RNA splicing and MYC-dependent vulnerability. EMBO Mol Med 10:N/A (2018). Read more (PubMed: 29769258) »
- Petsalaki E & Zachos G Clks 1, 2 and 4 prevent chromatin breakage by regulating the Aurora B-dependent abscission checkpoint. Nat Commun 7:11451 (2016). Read more (PubMed: 27126587) »
