Key features and details
- Rabbit polyclonal to CLK2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CLK2 antibody
See all CLK2 primary antibodies
DescriptionRabbit polyclonal to CLK2
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chinese hamster
Synthetic peptide corresponding to Human CLK2 aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab86147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionPhosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates serines, threonines and tyrosines.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.
Contains 1 protein kinase domain.
modificationsAutophosphorylates on all three types of residues.
- Information by UniProt
- CDC like kinase 2 antibody
- CDC-like kinase 2 antibody
- CLK 2 antibody
ab86147 staining CLK2 in human colon carcinoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and blocked with 1% BSA for 30 minutes at 37°C. Samples were incubated with primary antibody (1/30 in PBS + 1% BSA) for 16 hour at 4°C. A FITC-conjugated goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.
Anti-CLK2 antibody (ab86147) at 0.5 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 60 kDa
Additional bands at: 36 kDa, 64 kDa, 94 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
Human Dual specificity protein kinase CLK2 contains a number of potential phosphorylation sites (SwissProt) which may explain the banding pattern at a higher molecular weight than predicted (64 kDa).
ICC/IF image of ab86147 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab86147 at 5ug overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti- Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in HeLa pfa fixed cell types at 5ug/ml
ab86147 has been referenced in 2 publications.
- Iwai K et al. Anti-tumor efficacy of a novel CLK inhibitor via targeting RNA splicing and MYC-dependent vulnerability. EMBO Mol Med 10:N/A (2018). PubMed: 29769258
- Petsalaki E & Zachos G Clks 1, 2 and 4 prevent chromatin breakage by regulating the Aurora B-dependent abscission checkpoint. Nat Commun 7:11451 (2016). PubMed: 27126587