Overview

  • Product name
  • Description
    Rabbit polyclonal to CLK4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 107-156 (RSRRSSPKRK RNRHCSSHQS RSKSHRRKRS RSIEDDEEGH LICQSGDVLR) of Human CLK4 (NP_065717).

  • Positive control
    • Human fetal brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab104321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 57 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates SRSF1 and SRSF3. Required for the regulation of alternative splicing of MAPT/TAU. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.
  • Tissue specificity
    Expressed in liver, kidney, heart, muscle, brain and endothelial cells.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylates on all three types of residues.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI987988 antibody
    • C85119 antibody
    • CDC like kinase 4 antibody
    • CDC-like kinase 4 antibody
    • CLK4 antibody
    • CLK4_HUMAN antibody
    • DKFZp686A20267 antibody
    • Dual specificity protein kinase CLK4 antibody
    • OTTMUSP00000005756; antibody
    • OTTMUSP00000005757 antibody
    • OTTMUSP00000041113 antibody
    • Protein serine threonine kinase Clk4 antibody
    • RP23-9N7.1 antibody
    see all

Images

  • Anti-CLK4 antibody (ab104321) at 1 µg/ml + Human fetal brain lysate at 10 µg

    Predicted band size: 57 kDa



    Gel concentration: 12%
  • Sample type: Human microvascular endothelial cells (25µg) Primary Dilution (ab104321): 1:1000 Secondary Antibody: Goat anti-Rabbit-HRP Secondary Dilution: 1:5000

References

This product has been referenced in:

See all 2 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Colon carcinoma)
Permeabilization
No
Specification
Colon carcinoma
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C
Fixative
Methanol
Username

Dr. Eleni Petsalaki

Verified customer

Submitted Jul 16 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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