Overview

  • Product name

  • Description

    Mouse polyclonal to CLK4
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    CLK4 full length human protein.

  • Positive control

    • CLK4 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 57 kDa).

Target

  • Function

    Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates SRSF1 and SRSF3. Required for the regulation of alternative splicing of MAPT/TAU. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.
  • Tissue specificity

    Expressed in liver, kidney, heart, muscle, brain and endothelial cells.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylates on all three types of residues.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • AI987988 antibody
    • C85119 antibody
    • CDC like kinase 4 antibody
    • CDC-like kinase 4 antibody
    • CLK4 antibody
    • CLK4_HUMAN antibody
    • DKFZp686A20267 antibody
    • Dual specificity protein kinase CLK4 antibody
    • OTTMUSP00000005756; antibody
    • OTTMUSP00000005757 antibody
    • OTTMUSP00000041113 antibody
    • Protein serine threonine kinase Clk4 antibody
    • RP23-9N7.1 antibody
    see all

Images

  • All lanes : Anti-CLK4 antibody (ab67936) at 1/500 dilution

    Lane 1 : CLK4 transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size: 57 kDa
    Observed band size: 62 kDa
    why is the actual band size different from the predicted?

References

ab67936 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

You are welcome. I will gather the information we need for the CLK1 and CLK4 antibodies and will send it as soon as I receive it.

Read More

Answer

Thank you for your patience. None of our CLK antibodies have been tested against purified protein of other CLK family members. Cross-reactivity of, for instance, a CLK1 antibody with CLK2, 3, or 4 has not been confirmed. The best we can do is predict the potential for cross-reactivity by aligning the immunogens of each antibody, when they are available, with the sequences of the other proteins. The N terminus appears to have a lot of diversity among these four, so antibodies raised against the N terminus are likely to have specificity for their targets. For example, the CLK2 antibody ab86147 is raised against the CLK2 N terminus which has little or no homology with the N termini of CLK1, 3, or 4. This is also the case for the CLK3 antibody ab37919, raised against the N terminus of CLK3. We do not have the immunogen sequence information for the CLK1 and CLK4 antibodies in our catalogue, but I can obtain alignment scores for these if you are still interested. For proteins, we only have a CLK1 protein, ab114706, full-length recombinant human with a 26 kDa GST tag. There is also a CLK2 protein fragment, but it does not include the N terminus. I hope this helps, at least a little. Please let me know if you need more advice or information.

Read More

Answer

Thank you for contacting us. I am very sorry for the confusion. I performed a BLAST to find out the percentage of homology between the four proteins, and the results are: -CLK1-CLK2: 53.5% -CLK1-CLK3: 14% -CLK1-CLK4: 77% -CLK2-CLK3: 17% -CLK2-CLK4: 55% -CLK3-CLK4: 14% We usually predict cross-reactivity when the homology between the sequences is over 85%. So, in this case, I would dare to affirm there might be some slight reactivity between CLK1 and CLK4, but the rest of proteins are very unlikely to cross react. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up