Overview

  • Product name

    Anti-CLLD8/SETDB2 antibody
    See all CLLD8/SETDB2 primary antibodies
  • Description

    Goat polyclonal to CLLD8/SETDB2
  • Host species

    Goat
  • Specificity

    This antibody is expected to recognise both reported isoforms.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human CLLD8/SETDB2 aa 174-188 (N terminal) (Cysteine residue).
    Sequence:

    C-QRRHAKTNSHSSALH


    Database link: Q96T68

  • Positive control

    • Human heart lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 82 kDa).

Target

  • Function

    Histone methyltransferase involved in left-right axis specification in early development and mitosis. Specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). H3K9me3 is a specific tag for epigenetic transcriptional repression that recruits HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Contributes to H3K9me3 in both the interspersed repetitive elements and centromere-associated repeats. Plays a role in chromosome condensation and segregation during mitosis.
  • Tissue specificity

    Ubiquitous. Highest expression in heart, testis and ovary.
  • Sequence similarities

    Belongs to the histone-lysine methyltransferase family.
    Contains 1 MBD (methyl-CpG-binding) domain.
    Contains 1 pre-SET domain.
    Contains 1 SET domain.
  • Cellular localization

    Nucleus. Chromosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • C13orf4 antibody
    • Chromosome 13 open reading frame 4 antibody
    • Chronic lymphocytic leukemia deletion region 8 antibody
    • Chronic lymphocytic leukemia deletion region gene 8 antibody
    • Chronic lymphocytic leukemia deletion region gene 8 protein antibody
    • Clld8 antibody
    • CLLL8 antibody
    • Gm293 antibody
    • H3-K9-HMTase antibody
    • Histone H3-K9 methyltransferase antibody
    • Histone-lysine N-methyltransferase SETDB2 antibody
    • KMT1F antibody
    • Lysine N-methyltransferase 1F antibody
    • Probable histone-lysine N-methyltransferase H3 lysine-9 specific antibody
    • SEB2 antibody
    • SET domain bifurcated 2 antibody
    • SET domain protein, bifurcated, 2 antibody
    • SETB2_HUMAN antibody
    • Setdb2 antibody
    see all

Images

  • Anti-CLLD8/SETDB2 antibody (ab133174) at 0.1 µg/ml + Human heart cell lysate in RIPA buffer

    Developed using the ECL technique.

    Predicted band size: 82 kDa
    Observed band size: 90 kDa
    why is the actual band size different from the predicted?

References

ab133174 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab133174.
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