Anti-CLLD8/SETDB2 antibody (ab13712)
Key features and details
- Goat polyclonal to CLLD8/SETDB2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CLLD8/SETDB2 antibody
See all CLLD8/SETDB2 primary antibodies -
Description
Goat polyclonal to CLLD8/SETDB2 -
Host species
Goat -
Specificity
This antibody has been shown to detect SETDB2 in overexpression cell extracts. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig -
Immunogen
Synthetic peptide corresponding to Human CLLD8/SETDB2 aa 1-100 (N terminal). (NP_114121.2; NP_001153780.1)
Database link: Q96T68 -
Positive control
- WB: HeLa nuclear lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab13712 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 83 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 83 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Histone methyltransferase involved in left-right axis specification in early development and mitosis. Specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). H3K9me3 is a specific tag for epigenetic transcriptional repression that recruits HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Contributes to H3K9me3 in both the interspersed repetitive elements and centromere-associated repeats. Plays a role in chromosome condensation and segregation during mitosis. -
Tissue specificity
Ubiquitous. Highest expression in heart, testis and ovary. -
Sequence similarities
Belongs to the histone-lysine methyltransferase family.
Contains 1 MBD (methyl-CpG-binding) domain.
Contains 1 pre-SET domain.
Contains 1 SET domain. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 509382 Cow
- Entrez Gene: 83852 Human
- Entrez Gene: 239122 Mouse
- Entrez Gene: 100361710 Rat
- Omim: 607865 Human
- SwissProt: Q96T68 Human
- SwissProt: Q8C267 Mouse
- Unigene: 631789 Human
see all -
Alternative names
- C13orf4 antibody
- Chromosome 13 open reading frame 4 antibody
- Chronic lymphocytic leukemia deletion region 8 antibody
see all
Images
Datasheets and documents
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Datasheet download
References (2)
ab13712 has been referenced in 2 publications.
- Wang Z et al. CREB stimulates GPX4 transcription to inhibit ferroptosis in lung adenocarcinoma. Oncol Rep 45:N/A (2021). PubMed: 33846793
- Mu G & Chen F Oncogenic Roles Of A Histone Methyltransferase SETDB2 In AML1-ETO Positive AML. Cancer Manag Res 12:783-792 (2020). PubMed: 32099474