Overview

  • Product name
    Anti-CLLD8/SETDB2 antibody
    See all CLLD8/SETDB2 primary antibodies
  • Description
    Goat polyclonal to CLLD8/SETDB2
  • Host species
    Goat
  • Specificity
    This antibody has been shown to detect SETDB2 in overexpression cell extracts.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human CLLD8/SETDB2 aa 2-14 (N terminal).
    Sequence:

    GEKNGDAKTFWME-C


    Database link: Q96T68
    (Peptide available as ab23232)

  • General notes
    GenBank Accession Number - NP_114121

Properties

Applications

Our Abpromise guarantee covers the use of ab13712 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 83 kDa).Can be blocked with Human CLLD8/SETDB2 peptide (ab23232).

Target

  • Function
    Histone methyltransferase involved in left-right axis specification in early development and mitosis. Specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). H3K9me3 is a specific tag for epigenetic transcriptional repression that recruits HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Contributes to H3K9me3 in both the interspersed repetitive elements and centromere-associated repeats. Plays a role in chromosome condensation and segregation during mitosis.
  • Tissue specificity
    Ubiquitous. Highest expression in heart, testis and ovary.
  • Sequence similarities
    Belongs to the histone-lysine methyltransferase family.
    Contains 1 MBD (methyl-CpG-binding) domain.
    Contains 1 pre-SET domain.
    Contains 1 SET domain.
  • Cellular localization
    Nucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • C13orf4 antibody
    • Chromosome 13 open reading frame 4 antibody
    • Chronic lymphocytic leukemia deletion region 8 antibody
    • Chronic lymphocytic leukemia deletion region gene 8 antibody
    • Chronic lymphocytic leukemia deletion region gene 8 protein antibody
    • Clld8 antibody
    • CLLL8 antibody
    • Gm293 antibody
    • H3-K9-HMTase antibody
    • Histone H3-K9 methyltransferase antibody
    • Histone-lysine N-methyltransferase SETDB2 antibody
    • KMT1F antibody
    • Lysine N-methyltransferase 1F antibody
    • Probable histone-lysine N-methyltransferase H3 lysine-9 specific antibody
    • SEB2 antibody
    • SET domain bifurcated 2 antibody
    • SET domain protein, bifurcated, 2 antibody
    • SETB2_HUMAN antibody
    • Setdb2 antibody
    see all

Images

  • Anti-CLLD8/SETDB2 antibody (ab13712) at 0.5 µg/ml (Staining of Human Heart lysate)
    Predicted band size: 83 kDa

References

ab13712 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for your inquiry. I BLASTed the immunogen sequence of ab5517 against mouse and it only has 55% homology.  We generally say that anything with a greater than 85% homology would be expected to cross-react, so this is quite low.  This antibody probably wouldn't work with mouse samples. However, we do have a goat polyclonal anti-CLLD8/SETDB2 antibody that is predicted to cross-react with mouse, ab13712.  This immunogen is 100% homologous with mouse. However, since we have not tested ab13712 in mouse, we would not be able to guarantee that it would work. https://www.abcam.com/CLLD8-SETDB2-antibody-ab13712.html But, I can offer a discount off a future purchase if you buy ab13712 now, test it in mouse and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab13712 in mouse.  I will then send a discount code. This code must be issued before purchasing ab13712 so please wait for my reply before ordering. 2. Purchase ab13712 either by phone, fax, or online (www.abcam.com). 3. Test it in mouse. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab13712 turns out to be unsuitable for mouse, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.  

Read More

Answer

I'm very sorry to hear you are having problems with ab5517 and ab13712. You do not mention adding loading buffer to your samples prior to boiling, please make sure you add loading buffer containing SDS, DTT, beta-mercaptoethanol and bromphenol blue. Please also make sure you keep samples on ice until boiling and that protease inhibitors are added freshly. You do not mention how long you block the membrane for (and what temperature), I recommend trying 5%BSA in TBST for 1 hr, then rinsing a few seconds in TBST and incubating both primary and secondary antibodies in TBST. The dilution recommended for ab5517 is 1:5000 and for ab13712 is ug/ml but you may need to dilute more, I hope these suggestions help, please do not hesitate to contact us if you need further assistance,

Read More

Answer

Thank you for your enquiry. Currently we don't have either the lysate or the clone for this antibody within our catalogue. In the future, if you cannot find what you are looking for within the Abcam catalogue, you can click on the link to "The World's Antibody Gateway". The World's Antibody Gateway is a free search engine service provided by Abcam to help you to quickly find the antibodies that you are looking for. It is a free-text search engine developed by the Abcam team so that it searches the catalogues of all online antibody companies (currently 249). Should you require any further information, please get in touch with us and we will gladly assist you.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up