Anti-CLLD8/SETDB2 antibody (ab56184)
- Datasheet
- References (1)
- Protocols
Overview
-
Product name
Anti-CLLD8/SETDB2 antibody
See all CLLD8/SETDB2 primary antibodies -
Description
Rabbit polyclonal to CLLD8/SETDB2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ELISA, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human CLLD8/SETDB2 (N terminal).
Database link: Q96T68
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab56184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| ELISA | ||
| ICC/IF |
Application notes
ELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 µg/ml for 2hrs. Predicted molecular weight: 82 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 µg/ml for 2hrs. Predicted molecular weight: 82 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
-
Function
Histone methyltransferase involved in left-right axis specification in early development and mitosis. Specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). H3K9me3 is a specific tag for epigenetic transcriptional repression that recruits HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Contributes to H3K9me3 in both the interspersed repetitive elements and centromere-associated repeats. Plays a role in chromosome condensation and segregation during mitosis. -
Tissue specificity
Ubiquitous. Highest expression in heart, testis and ovary. -
Sequence similarities
Belongs to the histone-lysine methyltransferase family.
Contains 1 MBD (methyl-CpG-binding) domain.
Contains 1 pre-SET domain.
Contains 1 SET domain. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
-
Database links
- Entrez Gene: 83852 Human
- Omim: 607865 Human
- SwissProt: Q96T68 Human
- Unigene: 631789 Human
-
Alternative names
- C13orf4 antibody
- Chromosome 13 open reading frame 4 antibody
- Chronic lymphocytic leukemia deletion region 8 antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-CLLD8/SETDB2 antibody (ab56184)Image from Falandry C et al, J Biol Chem. 2010 Jun 25;285(26):20234-41. Epub 2010 Apr 19, Fig 1. DOI 10.1074/jbc.M109.052399ab56184 staining CLLD8/SETDB2 in 293T cells by Immunocytochemistry/ Immunofluorescence.
After 2–6 days, the cells grown on coverslips were washed and fixed with 4% paraformaldehyde for 10 minutes, permeabilized, and incubated with ab56184 at a 1/50 dilution for 1 hour at 37 °C. A secondary antibody directed against the rabbit primary antibody conjugated with Alexa-Fluor 488 was used for detection. The nuclei were counterstained with DAPI.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Falandry C et al. CLLD8/KMT1F is a lysine methyltransferase that is important for chromosome segregation. J Biol Chem 285:20234-41 (2010). WB, ICC/IF ; Human . Read more (PubMed: 20404330) »
