Anti-CLLD8/SETDB2 antibody (ab56184)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-CLLD8/SETDB2 antibody
See all CLLD8/SETDB2 primary antibodies -
DescriptionRabbit polyclonal to CLLD8/SETDB2
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide corresponding to Human CLLD8/SETDB2 (N terminal).
Database link: Q96T68
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.01% Sodium Azide
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab56184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| ELISA | ||
| ICC/IF |
Application notes
ELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 µg/ml for 2hrs. Predicted molecular weight: 82 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 µg/ml for 2hrs. Predicted molecular weight: 82 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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FunctionHistone methyltransferase involved in left-right axis specification in early development and mitosis. Specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). H3K9me3 is a specific tag for epigenetic transcriptional repression that recruits HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Contributes to H3K9me3 in both the interspersed repetitive elements and centromere-associated repeats. Plays a role in chromosome condensation and segregation during mitosis.
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Tissue specificityUbiquitous. Highest expression in heart, testis and ovary.
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Sequence similaritiesBelongs to the histone-lysine methyltransferase family.
Contains 1 MBD (methyl-CpG-binding) domain.
Contains 1 pre-SET domain.
Contains 1 SET domain. -
Cellular localizationNucleus. Chromosome.
- Information by UniProt
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Database links
- Entrez Gene: 83852 Human
- Omim: 607865 Human
- SwissProt: Q96T68 Human
- Unigene: 631789 Human
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Alternative names
- C13orf4 antibody
- Chromosome 13 open reading frame 4 antibody
- Chronic lymphocytic leukemia deletion region 8 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CLLD8/SETDB2 antibody (ab56184)Image from Falandry C et al, J Biol Chem. 2010 Jun 25;285(26):20234-41. Epub 2010 Apr 19, Fig 1. DOI 10.1074/jbc.M109.052399ab56184 staining CLLD8/SETDB2 in 293T cells by Immunocytochemistry/ Immunofluorescence.
After 2–6 days, the cells grown on coverslips were washed and fixed with 4% paraformaldehyde for 10 minutes, permeabilized, and incubated with ab56184 at a 1/50 dilution for 1 hour at 37 °C. A secondary antibody directed against the rabbit primary antibody conjugated with Alexa-Fluor 488 was used for detection. The nuclei were counterstained with DAPI.
Protocols
References
This product has been referenced in:
- Falandry C et al. CLLD8/KMT1F is a lysine methyltransferase that is important for chromosome segregation. J Biol Chem 285:20234-41 (2010). WB, ICC/IF ; Human . Read more (PubMed: 20404330) »
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"