Key features and details
- Rabbit polyclonal to CLN3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CLN3 antibody
DescriptionRabbit polyclonal to CLN3
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human CLN3 (internal sequence). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: Q13286
- WB: NTERA-2 cl.D1 [NT2/D1], Jurkat, Raji and NCI-H929 whole cell lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227592 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 48 kDa.|
FunctionInvolved in microtubule-dependent, anterograde transport of late endosomes and lysosomes.
Involvement in diseaseDefects in CLN3 are the cause of neuronal ceroid lipofuscinosis type 3 (CLN3) [MIM:204200]; also known as Batten disease. A form of neuronal ceroid lipofuscinosis. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy. The hallmark of CLN3 is the ultrastructural pattern of lipopigment with a fingerprint profile, which can have 3 different appearances: pure within a lysosomal residual body; in conjunction with curvilinear or rectilinear profiles; and as a small component within large membrane-bound lysosomal vacuoles. The combination of fingerprint profiles within lysosomal vacuoles is a regular feature of blood lymphocytes from patients with CLN3.
Sequence similaritiesBelongs to the battenin family.
Farnesylation is important for trafficking to lysosomes.
Cellular localizationLysosome membrane. Late endosome.
- Information by UniProt
- Batten disease protein antibody
- Battenin antibody
- BTS antibody
Anti-CLN3 antibody (ab227592) at 1/1000 dilution + NTERA-2 cl.D1 [NT2/D1] (human malignant pluripotent embryonic carcinoma cell line) whole cell lysate at 30 µg
HRP-conjugated anti-rabbit IgG
Predicted band size: 48 kDa
10% SDS-PAGE gel.
All lanes : Anti-CLN3 antibody (ab227592) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : NCI-H929 whole cell lysate
Lysates/proteins at 30 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 48 kDa
10% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227592 has not yet been referenced specifically in any publications.