Product nameAnti-CLN5 antibody [EPR12197(B)]
See all CLN5 primary antibodies
DescriptionRabbit monoclonal [EPR12197(B)] to CLN5
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Rat, Human
- Fetal kidney, T47-D and A431 lysates; Human kidney tissue; A431 cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab170899 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 41 kDa.|
|IHC-P||1/100 - 1/250.|
|ICC/IF||1/100 - 1/250.|
Involvement in diseaseDefects in CLN5 are the cause of neuronal ceroid lipofuscinosis type 5 (CLN5) [MIM:256731]; also known as Finnish variant late-infantile neuronal ceroid lipofuscinosis (vLINCL). A form of neuronal ceroid lipofuscinosis. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy. The lipopigment patterns observed most often in neuronal ceroid lipofuscinosis type 5 comprise mixed combinations of granular, curvilinear, and fingerprint profiles.
Sequence similaritiesBelongs to the CLN5 family.
- Information by UniProt
- Ceroid lipofuscinosis neuronal 5 antibody
- Ceroid-lipofuscinosis neuronal protein 5 antibody
- CLN5 antibody
All lanes : Anti-CLN5 antibody [EPR12197(B)] (ab170899) at 1/1000 dilution
Lane 1 : Fetal kidney lysate
Lane 2 : T47-D cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 41 kDa
Immunofluorescent analysis of A431 cells labeling CLN5 with ab170899 at 1/100 dilution (green). DAPI nuclear staining (blue).
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CLN5 with ab170899 at 1/100 dilution.
This product has been referenced in:
- Adams J et al. Autophagy-lysosome pathway alterations and alpha-synuclein up-regulation in the subtype of neuronal ceroid lipofuscinosis, CLN5 disease. Sci Rep 9:151 (2019). Read more (PubMed: 30655561) »
- Danyukova T et al. Loss of CLN7 results in depletion of soluble lysosomal proteins and impaired mTOR reactivation. Hum Mol Genet 27:1711-1722 (2018). Read more (PubMed: 29514215) »