Overview

  • Product name
    Anti-CLN5 antibody [EPR12197(B)]
    See all CLN5 primary antibodies
  • Description
    Rabbit monoclonal [EPR12197(B)] to CLN5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CLN5 aa 150-250 (Cysteine residue). The exact sequence is proprietary.
    Database link: O75503
    (Peptide available as ab204069)

  • Positive control
    • Fetal kidney, T47-D and A431 lysates; Human kidney tissue; A431 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR12197(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Tissue specificity
      Ubiquitous.
    • Involvement in disease
      Defects in CLN5 are the cause of neuronal ceroid lipofuscinosis type 5 (CLN5) [MIM:256731]; also known as Finnish variant late-infantile neuronal ceroid lipofuscinosis (vLINCL). A form of neuronal ceroid lipofuscinosis. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy. The lipopigment patterns observed most often in neuronal ceroid lipofuscinosis type 5 comprise mixed combinations of granular, curvilinear, and fingerprint profiles.
    • Sequence similarities
      Belongs to the CLN5 family.
    • Post-translational
      modifications
      Glycosylated.
    • Cellular localization
      Lysosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • Ceroid lipofuscinosis neuronal 5 antibody
      • Ceroid-lipofuscinosis neuronal protein 5 antibody
      • CLN5 antibody
      • CLN5_HUMAN antibody
      • NCL antibody
      • Protein CLN5 antibody
      see all

    Images

    • All lanes : Anti-CLN5 antibody [EPR12197(B)] (ab170899) at 1/1000 dilution

      Lane 1 : Fetal kidney lysate
      Lane 2 : T47-D cell lysate
      Lane 3 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 41 kDa

    • Immunofluorescent analysis of A431 cells labeling CLN5 with ab170899 at 1/100 dilution (green). DAPI nuclear staining (blue).

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CLN5 with ab170899 at 1/100 dilution.

    References

    This product has been referenced in:
    • Danyukova T  et al. Loss of CLN7 results in depletion of soluble lysosomal proteins and impaired mTOR reactivation. Hum Mol Genet 27:1711-1722 (2018). Read more (PubMed: 29514215) »
    • Flor AC  et al. A signature of enhanced lipid metabolism, lipid peroxidation and aldehyde stress in therapy-induced senescence. Cell Death Discov 3:17075 (2017). WB . Read more (PubMed: 29090099) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human fibroblast)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    100 µg
    Specification
    human fibroblast
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

    Dr. Stella Lee

    Verified customer

    Submitted Sep 23 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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