Key features and details
- Rabbit polyclonal to CLNS1A/CLCI - C-terminal
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-CLNS1A/CLCI antibody - C-terminal
See all CLNS1A/CLCI primary antibodies
DescriptionRabbit polyclonal to CLNS1A/CLCI - C-terminal
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Rabbit, Goat, Horse, Cow, Dog, Pig, Xenopus laevis, Chinese hamster, Orangutan
- HeLa, 293T, Jurkat, TCMK-1 and NIH3T3 whole cell lysates.
This product was previously labelled as CLNS1A
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab192907 was affinity purified using an epitope specific to CLNS1A/CLCI immobilized on solid support
Our Abpromise guarantee covers the use of ab192907 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/1000. Predicted molecular weight: 26 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
|IHC-P||1/1000 - 1/5000.|
|IF||1/500 - 1/2500.|
FunctionThe interaction with Sm proteins inhibits their assembly on U RNA and interferes with snRNP biogenesis. Inhibits the binding of survival motor neuron protein (SMN) to Sm proteins. May participate in cellular volume control by activation of a swelling-induced chloride conductance pathway.
Sequence similaritiesBelongs to the pICln family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton. A small fraction is also associated with the cytoskeleton.
- Information by UniProt
- Chloride channel antibody
- Chloride channel nucleotide sensitive 1A antibody
- Chloride channel regulatory protein antibody
ab192907 staining CLNS1A/CLCI in mouse tetratoma tissue sections. Primary antibody was used at a dilution of 1/1000.
Counterstain: Hematoxylin (blue).
ab192907 staining CLNS1A/CLCI in human ovarian carcinoma tissue sections. Primary antibody used at a dilution of 1/1000.
Counterstain: Hematoxylin (blue).
All lanes : Anti-CLNS1A/CLCI antibody - C-terminal (ab192907) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 26 kDa
Exposure time: 30 seconds
Lysates prepared using NETN lysis buffer.
Detection of CLNS1A/CLCI in immunoprecipitates of 293T whole cell lysate prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab192907 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
ab192907 has not yet been referenced specifically in any publications.