Overview

  • Product name

    Anti-Clostridium botulinum E Toxoid antibody [209F2]
  • Description

    Mouse monoclonal [209F2] to Clostridium botulinum E Toxoid
  • Host species

    Mouse
  • Specificity

    BoTox-E (2-17) 100%; BoTox-E 100% Does not cross react with BoTox-A, BoTox-B, BoTox-C or BoTox-F (1-16)
  • Tested applications

    Suitable for: ICC/IF, Inhibition Assaymore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Synthetic peptide analogue of the amino terminal region of BoTox-E.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Constituent: Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Purification notes

    Sterile filtered culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    209F2
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab40787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Inhibition Assay
  • Application notes
    IF: 1/50.
    This product has been found to stain Neuro2A cells treated with GT1b ganglioside prior to the addition of BoTox-E at a dilution of 1:50 using an indirect immunocytochemical staining procedure.

    Inhib: Use at an assay dependent dilution.
    This product has been found to inhibit the entry of BoTox-E to Neuro2A cells treated with GT1b ganglioside prior to the addition of the toxin.

    With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      Clostridium botulinum E Toxoid acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase. It has a non-toxic component which is necessary to maintain toxicity.
    • Cellular localization

      Secreted
    • Alternative names

      • BoNT/E antibody
      • Bontoxilysin e antibody
      • Botulinum neurotoxin type e non toxic component antibody
      • Botulinum neurotoxin type e precursor antibody
      • Botulism E toxoid antibody
      • Ent120 antibody
      see all

    References

    This product has been referenced in:

    • Shu Y  et al. Phosphorylation of SNAP-25 at Ser187 mediates enhancement of exocytosis by a phorbol ester in INS-1 cells. J Neurosci 28:21-30 (2008). Read more (PubMed: 18171919) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab40787.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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