Key features and details
- Mouse monoclonal [209F2] to Clostridium botulinum E Toxoid
- Suitable for: ICC/IF, Inhibition Assay
- Isotype: IgG
Product nameAnti-Clostridium botulinum E Toxoid antibody [209F2]
DescriptionMouse monoclonal [209F2] to Clostridium botulinum E Toxoid
SpecificityBoTox-E (2-17) 100%; BoTox-E 100% Does not cross react with BoTox-A, BoTox-B, BoTox-C or BoTox-F (1-16)
Tested applicationsSuitable for: ICC/IF, Inhibition Assaymore details
Species reactivityReacts with: Other species
Synthetic peptide analogue of the amino terminal region of BoTox-E.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferConstituent: Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Purification notesSterile filtered culture supernatant.
Our Abpromise guarantee covers the use of ab40787 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product has been found to stain Neuro2A cells treated with GT1b ganglioside prior to the addition of BoTox-E at a dilution of 1:50 using an indirect immunocytochemical staining procedure.
Inhib: Use at an assay dependent dilution.
This product has been found to inhibit the entry of BoTox-E to Neuro2A cells treated with GT1b ganglioside prior to the addition of the toxin.
With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceClostridium botulinum E Toxoid acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase. It has a non-toxic component which is necessary to maintain toxicity.
- BoNT/E antibody
- Bontoxilysin e antibody
- Botulinum neurotoxin type e non toxic component antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab40787 has been referenced in 1 publication.
- Shu Y et al. Phosphorylation of SNAP-25 at Ser187 mediates enhancement of exocytosis by a phorbol ester in INS-1 cells. J Neurosci 28:21-30 (2008). PubMed: 18171919